Modular assembly of protein building blocks to create precisely defined megamolecules.

Chembiochem

Departments of Chemistry and Biomedical Engineering, Howard Hughes Medical Institute, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.

Published: November 2012

Enzyme-promoted assembly: The construction of a hetero-bifunctional protein building block, HaloTag-cutinase, that reacts rapidly and selectively with a small-molecule linker is described. The step-wise combination of these building blocks generates a 300 kDa "megamolecule" with precisely defined domain orientation, connectivity, and composition.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804166PMC
http://dx.doi.org/10.1002/cbic.201200501DOI Listing

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