Enzyme-promoted assembly: The construction of a hetero-bifunctional protein building block, HaloTag-cutinase, that reacts rapidly and selectively with a small-molecule linker is described. The step-wise combination of these building blocks generates a 300 kDa "megamolecule" with precisely defined domain orientation, connectivity, and composition.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804166 | PMC |
http://dx.doi.org/10.1002/cbic.201200501 | DOI Listing |
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