Melanins are naturally occurring pigments in both normal and pathologic tissues. Two common bleaching processes are potassium permanganate followed by oxalic acid treatment and dilute hydrogen peroxide (H2O2) process. The potassium permanganate/oxalic acid method is faster and more easily incorporated in conventional daily immunostaining protocols, whereas the dilute H2O2 method requires 24 hours. This study aimed to reduce melanin bleaching time by using a 10% H2O2 dilution. First, reaction time was reduced to 30 minutes by raising the temperature to 65°C. Second, containers with high thermal conductivity were used to improve bleaching effectiveness. Experimental comparisons of melanin treatments with H2O2 contained in an iron jar, a glass coplin jar, and a plastic steel jar obtained bleaching time of 20, 30, and 40 minutes, respectively. These modifications of the conventional bleaching method significantly improve the speed and efficiency of the procedure and are recommended when performing immunohistochemical studies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/PAI.0b013e31826d81db | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of excessive bleaching on hair: comparative analysis of external morphology and internal microstructure.
Appl Microsc
December 2024
Department of Cosmetology, Hanseo University, Seosan, Chungnam, 31962, Republic of Korea.
This study investigates the impact of excessive bleaching on the external morphology and internal microstructure of hair, compared to untreated hair. Using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), we observed significant changes in both the surface and internal structures of bleached hair. SEM analysis of normal hair revealed a relatively clean surface with intact cuticle scales, while bleached hair showed brittle, torn scales with a rough appearance.
View Article and Find Full Text PDFAutomated Standardization of Melanin Bleaching Procedures of Heavily Pigmented Melanocytic Lesions With Low-Concentration Hydrogen Peroxide on an Automated Platform.
Appl Immunohistochem Mol Morphol
October 2024
Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital.
Melanin is a natural pigment in the human body that is primarily found in the skin and hair. It protects the skin from damage by ultraviolet radiation. Although this pigment plays a crucial role in protecting the human body, it represents a challenge for pathologists to evaluate highly pigmented tissue samples from melanoma or pigmented skin lesions.
View Article and Find Full Text PDFBiofilm-forming bacteria associated with corals secrete melanin with UV-absorption properties.
World J Microbiol Biotechnol
August 2024
Department of Science and Technology, Kavaratti, 682555, India.
Corals are colonized by a plethora of microorganisms, and their diversity plays a significant role in the health and resilience of corals when they face oxidative stress leading to bleaching. In the current study, we examined 238 bacteria isolated from five different coral species (Acropora hyacinthus, Pocillopora damicornis, Podabacea crustacea, Porites lobata, and Pavona venosa) collected from the coral reef ecosystems of Kavaratti, Lakshadweep Islands, India. We found that bacteria such as Psychrobacter sp.
View Article and Find Full Text PDFIn vitro efficacy of Rosa damascena solid state fermentation liquid and water extract on skin care.
Skin Res Technol
August 2024
Huzhou Jiaheng Industrial Co., Ltd., Huzhou, China.
Background: As a medicinal and food homologous plant, Rosa damascena is not only highly ornamental, but also rich in a variety of active ingredients such as polyphenols and flavonoids. It is widely used in cosmetics, food and pharmaceutical industries.
Objective: To study the in vitro efficacy of Rosa damascena solid state fermentation liquid (RDF) and water extract (RDE).
Structural and Evolutionary Relationships of Melanin Cascade Proteins in Cnidarian Innate Immunity.
Integr Comp Biol
November 2024
Department of Biology, University of Texas at Arlington, Arlington, TX 76019, USA.
Melanin is an essential product that plays an important role in innate immunity in a variety of organisms across the animal kingdom. Melanin synthesis is performed by many organisms using the tyrosine metabolism pathway, a general pathway that utilizes a type-three copper oxidase protein, called PO-candidates (phenoloxidase candidates). While melanin synthesis is well-characterized in organisms like arthropods and humans, it is not as well-understood in non-model organisms such as cnidarians.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!