AI Article Synopsis

  • A quick method using reversed-phase ion-pair HPLC with UV detection was created to separate polyprenyl phosphate oligomers on a regular C(18) column.
  • The method involves using tetrabutylammonium phosphate in an isocratic mode for effective results.
  • It was successfully used to analyze polyprenyl phosphates extracted from mulberry leaves and fir needles, which are key ingredients in certain veterinary drugs.

Article Abstract

A simple and rapid method of reversed-phase ion-pair high-performance liquid chromatography (HPLC) with UV-detection on a common C(18) column in an isocratic mode with the addition of tetrabutylammonium phosphate for the separation of polyprenyl phosphate oligomerhomologues has been developed. The method was successfully applied to assay the composition of polyprenyl phosphates obtained from polyprenols isolated from mulberry (Morus alba) leaves and fir (Abies sibirica) needles (the active components of veterinary drugs Gamapren and Phosprenyl correspondingly).

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Source
http://dx.doi.org/10.2116/analsci.28.1021DOI Listing

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