AI Article Synopsis
- Influenza virus likely assembles in specific areas of the plasma membrane known as raft domains, although earlier conclusions were debated due to varying experimental methods.
- Advanced fluorescence microscopy techniques, including FPALM, FRET, and FRAP, have enhanced our understanding of how viral proteins like HA and M2 interact with lipid domains.
- The findings contribute to the current model of virus assembly and reveal how the signals affecting lipid domain associations can influence the replication of the influenza virus in transfected cells.
Article Abstract
Influenza virus is thought to assemble in raft domains of the plasma membrane, but many of the conclusions were based on (controversial) Triton extraction experiments. Here we review how sophisticated methods of fluorescence microscopy, such as FPALM, FRET and FRAP, contributed to our understanding of lipid domain association of the viral proteins HA and M2. The results are summarized in light of the current model for virus assembly and lipid domain organization. Finally, it is described how the signals that govern domain association in transfected cells affect replication of influenza virus.
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| http://dx.doi.org/10.1111/cmi.12045 | DOI Listing |
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