AI Article Synopsis
- Wnt/β-catenin signaling is crucial for various cellular functions, particularly in promoting cell survival, though its specific mechanisms are not fully understood.
- Signal transducer and activator of transcription protein 3 (STAT3) plays a key role in regulating anti-apoptotic gene expression and is influenced by growth factors and cytokines.
- This study found that Wnt3a activation in the ARPE-19 cell line enhances cell viability under oxidative stress by increasing STAT3 activation, and reducing STAT3 levels negates the protective effects of Wnt3a, suggesting a significant link between Wnt3a signaling and STAT3 in supporting cell survival.
Article Abstract
Wnt/β-catenin signaling is an essential pathway that regulates numerous cellular processes, including cell survival. The molecular mechanisms contributing to pro-survival Wnt signaling are mostly unknown. Signal transducer and activator of transcription proteins (STATs) are a well-described family of transcription factors. STAT3 induces expression of anti-apoptotic genes in many tissues and is a downstream mediator of protective growth factors and cytokines. In this study, we investigated whether pro-survival Wnt signaling is mediated by STAT3. The Wnt3a ligand activated Wnt signaling in the retinal pigment epithelium ARPE-19 cell line and significantly increased the viability of cells exposed to oxidative stress. Furthermore, Wnt3a increased STAT3 activation and nuclear translocation, as measured by an antibody against phosphorylated STAT3. Reducing STAT3 levels with siRNA eliminated Wnt3a-dependent protection from oxidative stress. Together, these data demonstrate a previously unknown link between Wnt3a-mediated activation of STAT3 and cell survival, and indicate cross-talk between two important pro-survival signaling pathways.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3464242 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046892 | PLOS |
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