Activity profiling of the n-BuOH extract from Cimicifuga heracleifolia rhizomes led to the identification of three cytotoxic caffeic acid derivatives, carboxymethyl isoferulate (2), cimicifugic acid A (3), and cimicifugic acid B (4) together with a series of structurally related inactive compounds. The extract was separated by time-based fractionation in a gradient HPLC condition, and cytotoxicity of each fraction was evaluated using HCT116 colon cancer cells in vitro. HPLChyphenated spectroscopy including LC/NMR and LC/PDA/MS provided structural information for phenolic compounds contained in the extract, and further preparative isolation of active compounds 2-4 was achieved by semi-preparative HPLC. Compounds 2-4 showed cytotoxic activity against cancer cells in a dose-dependent manner at the concentrations of 2.5-40 μM, and western blotting analysis showed that these compounds increased expression of cleaved poly ADP ribose polymerase (PARP), a critical apoptosis marker.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12272-012-0906-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Quantitative Ethnobotany of Medicinal Plants from Darjeeling District of West Bengal, India, along with Phytochemistry and Toxicity Study of Buch.-Ham. ex D.Don bark.
Plants (Basel)
December 2024
Ethnopharmacology Laboratory, Department of Botany, Visva-Bharati University, Santiniketan 731235, West Bengal, India.
This study offers considerable information on plant wealth of therapeutic importance used traditionally by the residents of 11 villages under three subdivisions of Kurseong, Darjeeling Sadar, and Mirik in the Darjeeling District, West Bengal. For the acquisition of ethnomedicinal information, semi-structured interviews were conducted with 47 informants, of whom 11 persons were herbalists and 36 were knowledgeable persons. Free prior informed consent was obtained from each participant prior to the collection of field data.
View Article and Find Full Text PDFWilld., Source of Antioxidants with Diverse Chemical Structures.
Molecules
December 2024
Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna Street 12, 31-343 Kraków, Poland.
Ethanolic extracts from the roots and aerial parts of the hitherto chemically uninvestigated lettuce species Willd. (Cichorieae, Asteraceae) were chromatographically separated to obtain eight sesquiterpenoids, two apocarotenoids (loliolide and (6,9) roseoside), and three phenolic glucosides (apigenin 7--glucoside, eugenyl-4---glucopyranoside, and 5-methoxyeugenyl-4---glucopyranoside). Four of the isolated sesquiterpene lactones (8--angeloyloxyleucodin, matricarin, 15-deoxylactucin, and deacetylmatricarin 8--glucopyranoside) have not previously been found either in spp.
View Article and Find Full Text PDFEfficacy of natural NF-κB inhibitors in the treatment of fibrosarcoma: an in vitro model study.
Front Cell Dev Biol
December 2024
Department of Molecular and Cellular Biology, Faculty of Pharmacy, Wroclaw Medical University, Wroclaw, Poland.
Introduction: NF-κB plays a pivotal role in the progression of cancers, including myosarcomas such as fibrosarcoma. Plants possess considerable potential for the provision of chemotherapeutic effects against cancer. The present study assessed, among others, the cytotoxicity, migration capacity and DNA damage induced by several natural compounds (berberine, curcumin, biochanin A, cucurbitacin E (CurE) and phenethyl caffeic acid (CAPE)) in cancer cells (WEHI-164) and normal muscle cells (L6).
View Article and Find Full Text PDFAgro-morphological characterization and assessment of metabolic profiling and anticancer activities in various tribulus (Tribulus terrestris L.) populations.
BMC Plant Biol
January 2025
Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, 38156-8-8349, Iran.
Tribulus terrestris L. from the family of Zygophyllaceae, which is rich in saponin compounds, especially diosgenin, has various biological properties, such as anti-inflammation, anti-Alzheimer, anti-obesity, anti-diabetes, anti-leukemia, and anti-cancer activities, due to these compounds. This research aimed to study the diversity of agro-morphological and phytochemical traits and anti-proliferative activity against human prostate cancer cells (PC3) of T.
View Article and Find Full Text PDFEvaluation of the antimicrobial and anticancer potential of a modified silver nanoparticle-impregnated carrier system.
J Microencapsul
December 2024
Department of Aircraft Airframe Engine Maintenance, Kapadokya University, Nevşehir, Turkey.
This study aimed to develop silver nanoparticles embedded in poly(ricinoleic acid)-poly(methyl methacrylate)-poly(ethylene glycol) (AgNPsPRici-PMMA-PEG) nanoparticles (NPs) containing caffeic acid (Caff) and tetracycline hydrochloride (TCH) for treating infections and cancer in bone defects. The block copolymers were synthesised via free radical polymerisation. NPs were prepared using the solvent evaporation method and characterised by FTIR, HNMR, SEM, DSC, TGA, and DLS.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!