AI Article Synopsis
- The study investigates lipid metabolism in oocytes and embryos, which is often overlooked in human IVF.
- Researchers measured total carnitine content in follicular fluid from 278 IVF patients and found no correlation with estradiol levels or IVF outcomes.
- The study suggests that carnitine is important for lipid metabolism in oocytes, and its addition in maturation and culture media should be considered.
Article Abstract
Purpose: The purpose of this study is to study lipid metabolism in oocytes and embryos that is a neglected parameter in human IVF.
Methods: We have tested the total carnitine content (TC) in the follicular fluid of 278 patients (217 non pregnant, 61 pregnant) undergoing IVF.
Results: The follicular fluid TC is neither correlated with the circulating estradiol content in serum nor with the outcome the IVF attempt. Carnitine, through the carnitine shuttle, is a major partner in lipid beta oxidation, metabolic pathway involved in the acquisition of oocyte competence. The expression of carnitine synthesis enzymes and lipid beta oxidation was studied in cumulus cells collected at the time of ovum retrieval and in oocyte. Surprisingly the expression for carnitine synthesis is not detectable in oocytes whereas the enzymes involved in lipid beta oxidation are rather strongly expressed.
Conclusions: The addition of carnitine in oocyte maturation and embryo culture media should not be overlooked.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510375 | PMC |
| http://dx.doi.org/10.1007/s10815-012-9855-2 | DOI Listing |
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