AI Article Synopsis
- Teratoma tumor formation is vital for assessing the pluripotency of human pluripotent stem cells, but there's no consistent testing method currently.
- A new detailed teratoma assay involves transplanting undifferentiated human embryonic stem cells (hESCs) with inactive feeder cells into immunodeficient mice, showing high efficiency and reproducibility.
- The assay is sensitive enough to detect teratoma-forming cells even in differentiated cell populations, laying the groundwork for a standardized method for both pluripotency and bio-safety evaluations.
Article Abstract
Teratoma tumor formation is an essential criterion in determining the pluripotency of human pluripotent stem cells. However, currently there is no consistent protocol for assessment of teratoma forming ability. Here we present detailed characterization of a teratoma assay that is based on subcutaneous co-transplantation of defined numbers of undifferentiated human embryonic stem cells (hESCs) with mitotically inactivated feeder cells and Matrigel into immunodeficient mice. The assay was highly reproducible and 100% efficient when 100,000 hESCs were transplanted. It was sensitive, promoting teratoma formation after transplantation of 100 hESCs, though larger numbers of animals and longer follow-up were required. The assay could detect residual teratoma forming cells within differentiated hESC populations however its sensitivity was decreased in the presence of differentiated cells. Our data lay the foundation, for standardization of a teratoma assay for pluripotency analysis. The assay can also be used for bio-safety analysis of pluripotent stem cell-derived differentiated progeny.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3458078 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0045532 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Non-Invasive miRNA Profiling for Differential Diagnosis and Prognostic Stratification of Testicular Germ Cell Tumors.
Genes (Basel)
December 2024
Department of Biochemistry & Biotechnology, School of Health Sciences, University of Thessaly, 41335 Larissa, Greece.
Background/objectives: Testicular germ cell tumors (TGCT) are common in young adult men and have high cure rates. Conventional serum tumor markers and imaging are not able to differentiate between histologic subtypes of the disease, which portend different prognoses and require distinct therapeutic strategies. Micro-RNAs (miRNAs) are small non-coding transcripts involved in the post-transcriptional regulation of gene expression, which have emerged as promising biomarkers in a variety of tumors.
View Article and Find Full Text PDFMalignant transformation of a mature teratoma in the duodenum: A case report.
Int J Surg Case Rep
January 2025
Department of Gastro-intestinal Surgery, University Medical Center Ho Chi Minh City, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Viet Nam; Department of General Surgery, Faculty of Medicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Viet Nam.
Introduction: Mature teratomas of the duodenum are extremely rare, with only five cases reported in the English literature and none documented in Vietnam. Malignant transformation within a mature teratoma presents a challenging scenario for diagnosis and management, requiring a nuanced approach to treatment.
Presentation Of Case: We report the first documented case of malignant transformation in a duodenal mature teratoma in Vietnam.
Proposal of a novel index in assessing perinatal mortality in prenatal diagnosis of Sacrococcygeal Teratoma.
J Perinat Med
January 2025
Fetal Medicine Unit, Grupo CERAS, Clinica Anglo Americana, British Medical Hospital, Lima, Peru.
Objectives: To describe obstetric characteristics and perinatal outcomes in a serie of fetuses with Sacrococcygeal Teratoma (SCT) and propose a novel index to assess postnatal mortality based on the THC ratio and the addition of the presence of polyhydramnios.
Methods: A retrospective study in a referral teaching hospital between 2013 and 2023. A descriptive analysis and a receiver operating characteristic (ROC) curve were performed to the determine the optimal cutoff value of the THC plus polyhydramnios based on optimal sensitivity and specificity.
Comprehensive diagnostic and therapeutic approaches to uterine teratoma in postmenopausal women: a case study and review.
Front Oncol
December 2024
Department of Obstetrics and Gynecology, Dongguan Maternal and Child Health Care Hospital, Dongguan, China.
Article Synopsis
- Uterine teratomas are rare tumors found in postmenopausal women, and a case study of a 54-year-old woman highlights the challenges in diagnosing and treating them.
- Hysteroscopic surgery was used to successfully remove a mass previously thought to be a fibroid, which was later confirmed to be a benign teratoma containing diverse tissues like fat, hair, and cartilage.
- The study emphasizes the importance of hysteroscopy for diagnosis and treatment, discusses the difficulties in recognizing these tumors, and aims to enhance clinical understanding and guidelines for managing rare uterine masses.
Anti-N-methyl-D-aspartate (NMDA) receptor encephalitis is a rare autoimmune disorder that typically presents with neuropsychiatric symptoms and medial temporal lobe involvement. We report the case of a 24-year-old female with no significant medical history, who developed severe anxiety, memory deficits, and confusion over a two-week period. Neurological examination revealed cognitive dysfunction, asymmetric limb movements, and psychosis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!