The level of DNA damage and cytochrome c content in human lymphocytes in the dynamics of apoptosis induced by UV-light (240-390 nm) at doses of 151, 1510 and 3020 J/m2 is studied. DNA fragmentation is revealed in 20 h after UV-irradiation of lymphocytes at doses mentioned above. It is shown that DNA damages (single strand breaks) appear immediately after UV-irradiation of lymphocytes at doses of 1510 and 3020 J/m2 (comets of C1 type) and reach their maximum 6 h after cell modification (comets of C2 and C3 types). It is concluded that p53-dependent and receptor caspase pathways are involved in apoptosis development in the human lymphocytes, modified after UV-irradiation.
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