Viable Bordetella pertussis isolates are essential for surveillance purposes. We performed culture of 223 PCR-positive nasopharyngeal samples. B. pertussis was recovered from 45 (20.2%) of the samples. Growth was associated with a high bacterial load, as determined by PCR. Culture from PCR-positive samples is a feasible approach to recover B. pertussis isolates, and culture can be limited to samples with a high bacterial load.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502974 | PMC |
| http://dx.doi.org/10.1128/JCM.01553-12 | DOI Listing |
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