Am J Bot
Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 310018, People's Republic of China.
Published: October 2012
Premise Of The Study: Expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers were developed in Dendrobium officinale by screening a cDNA library. The loci were verified by sequencing and explored for polymorphism among 19 genotypes and transferability among 30 other distantly related Dendrobium species. •
Methods And Results: One hundred ten EST-SSRs were developed, and a set of 20 amplified two to six nucleotide repeats with a mean number of 2.5 alleles per locus and with an observed heterozygosity and polymorphism information content per locus ranging from 0.3463 to 0.9003 and 0.0997 to 0.6537 in 19 D. officinale genotypes, respectively. Furthermore, 92 of these markers have cross-taxa transferability, ranging from 6.45% to 100% among 30 other distantly related Dendrobium species. •
Conclusions: The developed markers have potential for application in germplasm appraisal, genetic diversity study, genetic mapping, and molecular breeding in D. officinale and other congeneric species.
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http://dx.doi.org/10.3732/ajb.1200132 | DOI Listing |
Food Sci Nutr
January 2025
Guangdong-Hong Kong-Macao Joint Laboratory for New Drug Screening, School of Pharmacy The Chinese University of Hong Kong Hong Kong SAR P. R. China.
is a kind of popular functional food to be consumed by both healthy and diabetic people. As its major constituent, polysaccharide (DOP) is mainly utilized by gut microbiota. Despite distinctive gut microbiota composition between healthy and diabetic individuals, no study compared the interplay between DOP and gut microbiota under healthy and diabetic status.
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January 2025
Changchun University of Chinese Medicine, Key Laboratory of Ginseng Efficacy Substance Base and Biological Mechanism Research, Ministry of Education, Changchun 130117, China; Northeast Asia Research Institute of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun 130117, China. Electronic address:
A large number of by-products generated in the food industry is discarded as waste, especially the residue left after extracting plant resources, which is typically repurposed as fertilizer. In this study, we extracted and purified a new protein, DOP1, from the residue of Dendrobium officinale Kimura & Migo (D. officinale), and explored the protective effect of DOP1 on alcohol-induced gastric mucosal injury.
View Article and Find Full Text PDFZhongguo Zhong Yao Za Zhi
October 2024
Jiangxi University of Chinese Medicine Nanchang 330004, China.
This study aims to mine the genes of Dendrobium officinale in response to high temperature stress and explore the molecular mechanism underlying the heat tolerance of D. officinale. In this study, the D.
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December 2024
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, People's Republic of China. Electronic address:
In this study, a homogeneous polysaccharide, named YDOP-1 was isolated form Dendrobium officinale using hot water extraction and ethanol precipitation method. YDOP-1 was characterized to be a typical O-acetyl-glucomannan with the molecular wight was 13,456 Da. Cell viability and colony forming assay showed that YDOP-1 possess moderate anti-gastric cancer effects.
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December 2024
The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, Hubei, China.
Background: Sugar transporters (STs) play a critical role in the transportation of sugar, participating in plant growth and development, stress/defense responses, and signal transduction processes. Dendrobium catenatum (also known as Dendrobium officinale, hereinafter referred to as D. catenatum) is an important traditional Chinese medicinal herb with remarkable medicinal properties and possessing high economic value.
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