The development of a competitive ELISA for the detection of brucella-specific antibodies in bovines is described. Anti-brucella guinea pig serum was used as a source of competing antibodies. Lipo-polysaccharide purified from inactivated B. abortus S19 culture was used as antigen for the development of the assay. Sera from cattle were used in the competitive ELISA, rose bengal test and a commercial indirect ELISA. The following cattle sera were tested: (i) known positive sera (n = 80) (ii) known negative sera (n = 100) and (iii) field sera (n = 1184). Based on the receiver operating characteristics curve analysis and frequency distribution of the percentage of inhibition, 30% inhibition was considered the cut-off for positive and negative results. The sensitivity and specificity estimate on comparison with the commercial indirect ELISA was 94.87 and 92.12% respectively. The competitive ELISA described is a simple method for the routine screening of animal sera for detecting Brucella-specific antibodies.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209930 | PMC |
| http://dx.doi.org/10.1007/s12088-011-0151-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Background: The key advantage of active immunization is the induction of sustained, polyclonal antibody responses that are readily boosted by occasional immunizations. Recent clinical trial outcomes for monoclonal antibodies lecanemab and donanemab, establish the relevance of targeting pathological Abeta for clearing amyloid plaques in Alzheimer's disease. ACI-24.
View Article and Find Full Text PDFPreparation and application of porcine broadly neutralizing monoclonal antibodies in an immunoassay for efficiently detecting neutralizing antibodies against foot-and-mouth disease virus serotype O.
Microbiol Spectr
January 2025
State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Neutralizing antibodies provide vital protection against foot-and-mouth disease virus (FMDV). The virus neutralization test (VNT) is a gold standard method for the detection of neutralizing antibodies. However, its application is limited due to the requirement for live virus and unsuitability for large-scale serological surveillance.
View Article and Find Full Text PDFChallenges of BTV-Group Specific Serology Testing: No One Test Fits All.
Viruses
November 2024
The Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australian Animal Health Laboratory, Australian Centre for Disease Preparedness, 5 Portarlington Road, East Geelong, VIC 3219, Australia.
A newly formatted enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to bluetongue virus (BTV) was developed and validated for bovine and ovine sera and plasma. Validation of the new sandwich ELISA (sELISA) was achieved with 949 negative bovine and ovine sera from BTV endemic and non-endemic areas of Australia and 752 BTV positive (field and experimental) sera verified by VNT and/or PCR. The test diagnostic sensitivity (DSe) and diagnostic specificity (DSp) were 99.
View Article and Find Full Text PDFSerological Detection of Ovine Gammaherpesvirus 2 Antibodies in Dairy Farms from Southern Brazil.
Microorganisms
December 2024
Moredun Research Institute, Pentlands Science Park, Midlothian, Edinburgh EH26 0PZ, UK.
Sheep-associated malignant catarrhal fever (SA-MCF) is a severe lymphoproliferative vascular disease of cattle that is caused by ovine gammaherpesvirus 2 (OvGHV2), which is a within the subfamily. SA-MCF occurs worldwide in several mammalian hosts. Alternatively, alcelaphine gammaherpesvirus 1 (AlGHV1) is a that causes wildebeest-associated malignant catarrhal fever (MCF), which principally occurs in cattle from Africa.
View Article and Find Full Text PDFNanobody-based indirect competitive ELISA for the detection of aflatoxin M1 in dairy products.
Sci Rep
January 2025
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Aflatoxin M1 (AFM1) is known to be carcinogenic, mutagenic, and teratogenic and poses a serious threat to food safety and human health, which makes its surveillance critical. In this study, an indirect competitive ELISA (icELISA) based on a nanobody (Nb M4) was developed for the sensitive and rapid detection of AFM1 in dairy products. In our previous work, Nb M4 was screened from a Bactrian-camel-immunized phage-displayed library.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!