Methylocystis parvus OBBP is an obligate methylotroph considered the type species of the genus Methylocystis. Two pmoCAB particulate methane monooxygenase operons and one additional singleton pmoC paralog were identified in the sequence. No evidence of genes encoding soluble methane monooxygenase was found. Comparison of M. parvus OBBP and Methylocystis sp. strain Rockwell (ATCC 49242) suggests that both species should be taxonomically classified in different genera.
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http://dx.doi.org/10.1128/JB.01346-12 | DOI Listing |
Microbiol Resour Announc
April 2023
BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, Biodiscovery Institute, University of Nottingham, Nottingham, United Kingdom.
A complete genome is presented for Methylocystis parvus OBBP, a Gram-negative aerobic methanotroph of the phylum . OBBP is the genus type strain and of interest in the production of polyhydroxybutyrate and environmental microbiology. The genome consists of two plasmids (248 kbp and 205 kbp) and a chromosome (4.
View Article and Find Full Text PDFACS Synth Biol
February 2023
BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, University of Nottingham, University Park, Nottingham NG7 2RD, U.K.
Methanotrophic bacteria are Gram-negative, aerobic organisms that use methane as their sole source of carbon and energy. In this study, we constructed and exemplified a CRISPR/Cas9 genome editing system and used it to successfully make gene deletions and insertions in the type I methanotroph Bath and the type II methanotroph OBBP. High frequencies of gene deletions and insertions were achieved in combination with homology-directed repair.
View Article and Find Full Text PDFACS Sustain Chem Eng
October 2022
Department of Engineering, University of Campania "Luigi Vanvitelli", Via Roma 29, 81031Aversa, Italy.
Bacterially produced polyhydroxyalkanoates are valuable substitutes for petrochemical plastics, but their current production capacities are very scarce. Producing poly(3-hydroxybutyrate--3-hydroxyvalerate) (PHB--HV) from methane and odd-chain carbon fatty acids could make the production of this biodegradable polymer cost-effective. This study analyzes the main factors affecting methanotrophic growth and poly(3-hydroxybutyrate--3-hydroxyvalerate) accumulation, simulating a pilot-scale process based on a double-stage approach.
View Article and Find Full Text PDFMicrobiol Spectr
June 2022
School of Civil and Environmental Engineering, Cornell Universitygrid.5386.8, Ithaca, New York, USA.
Methanotrophs, which help regulate atmospheric levels of methane, are active in diverse natural and man-made environments. This range of habitats and the feast-famine cycles seen by many environmental methanotrophs suggest that methanotrophs dynamically mediate rates of methane oxidation. Global methane budgets require ways to account for this variability in time and space.
View Article and Find Full Text PDFBioresour Technol
June 2022
Institute of Sustainable Processes, Universidad de Valladolid, Doctor Mergelina s/n, 47011, Spain. Electronic address:
Methylocystis parvus OBBP accumulates polyhydroxybutyrate (PHB) using methane as the sole carbon and energy source. In this work, the feasibility of producing (R)-3-hydroxybutyric acid (R3HBA) via intracellularly accumulated PHB through depolymerization (in-vivo) was investigated. Results showed that a PHB to R3HBA conversion of 77.
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