The Mouse P19 cell line was derived from an embryonal carcinoma. The pluripotent P19 cells can be induced to differentiate into neuronal and glial cells. Here, we describe an miRNA microarray method to monitor the miRNA expression profiles during the course of P19 cells neuronal differentiation. The results indicated that there were significant differences between the miRNA expression in P19 EC cells and the resultant differentiated neural stem cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-62703-083-0_9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Expression variation of long noncoding RNAs in dopaminergic cells-derived from stem cells and their MPP+ induced PD models.
Hum Mol Genet
January 2025
Department of Histology & Embryology, Rasht - Parastar Street, Guilan University of Medical Sciences, 13111-41937, Iran.
Parkinson's disease (PD) is the second most prevalent neurodegenerative disorder characterized by the progressive loss of nigrostriatal dopaminergic neurons (DA) which can be caused by environmental and genetic factors. lncRNAs have emerged as an important regulatory layer in neurodegenerative disorders, including PD. In this study, we investigated and validated lncRNAs that may serve as diagnostic or therapeutic targets for PD.
View Article and Find Full Text PDFKir4.1 and Aqp4 Contribution to Schisis Cystic Water Accumulation and Clearance in the Rs1 Exon-1 Del XLRS Rat Model.
Genes (Basel)
December 2024
Department of Ophthalmology, Eye Center, UC Davis School of Medicine, University of California Davis, Sacramento, CA 95817, USA.
Background/objective: The Rs1 exon-1-del rat (Rs1KO) XLRS model shows normal retinal development until postnatal day 12 (P12) when small cystic spaces start to form in the inner nuclear layer. These spaces enlarge rapidly, peak at P15, and then collapse by P19.
Methods: We explored the possible involvement of Kir4.
Analyzing exosomal miRNA profiles in tetralogy of fallot fetuses' amniotic fluid.
Sci Rep
January 2025
Department of Ultrasound, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, Fujian, China.
Amniotic fluid (AF)-derived exosomal miRNA have been explored as potential contributors to the pathogenesis of Tetralogy of Fallot (TOF). This study aimed to investigate the expression profiles of AF-derived exosomal miRNAs and their potential contribution to TOF development. Exosomes were isolated from AF samples obtained from pregnant women carrying fetuses diagnosed with TOF.
View Article and Find Full Text PDFAims: Sacubitril/valsartan (Sac/Val) is used for treatment of heart failure. The effect of Sac/Val on regional dysfunction following myocardial infarction (MI) remains uncertain. This study aimed at understanding the effects of Sac/Val on regional function after MI.
View Article and Find Full Text PDFExamining the NEUROG2 lineage and associated gene expression in human cortical organoids.
Development
January 2025
Sunnybrook Research Institute, Biological Sciences Platform, Hurvitz Brain Sciences Program, 2075 Bayview Ave, Toronto, ON M4N 3M5, Canada.
Proneural genes are conserved drivers of neurogenesis across the animal kingdom. How their functions have adapted to guide human-specific neurodevelopmental features is poorly understood. Here, we mined transcriptomic data from human fetal cortices and generated from human embryonic stem cell-derived cortical organoids (COs) to show that NEUROG1 and NEUROG2 are most highly expressed in basal neural progenitor cells, with pseudotime trajectory analyses indicating that NEUROG1-derived lineages predominate early and NEUROG2 lineages later.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!