Ethnopharmacology Relevance: Croton zehntneri is a Euphorbiaceae species native to northeastern Brazil, where teas and beverages made from Croton zehntneri leaves are used as healing agents. To our knowledge, there is no experimental study supporting this claim of pharmacological activity.
Materials And Methods: Full-thickness excisional wounds were made in the left and right sides of the dorsum of anesthetised Swiss mice, and a topical pharmaceutical formulation, developed by including essential oil extracted from the leaves of Croton zehntneri (2% and 20% EOCz) in pluronic-127 (PF-127), was administered to mice twice daily for 15 days post-wounding. To evaluate the contribution of trans-anethole, the major constituent of EOCz (85.7%), in the wound healing activity of EOCz, the effect of the topical administration of trans-AT on wound tissue repair was also evaluated and compared to other groups. A macroscopic analysis of swelling and exudates was performed and scored as 0 (missing), 1 (light), 2 (moderate) and 3 (intense). The number of capillaries and leukocytes was counted in hematoxylin and eosin (HE)-stained sections of the injured tissue. For extracellular matrix remodelling analysis, fibroblasts and collagen fibres present in the photomicrography of the Masson's Trichrome (MT)-stained sections were counted. Each experimental group comprised six mice.
Results: At day 3 post-wounding, it was observed that treatment with 20% EOCz greatly reduced the swelling and exudates with a similar magnitude to the dexamethasone treatment. The inflammatory cell infiltration and angiogenesis were not altered by either the EOCz- or trans-AT treatments. In contrast, an acceleration of the wound closure was observed, with an enhanced number of fibroblasts and collagen fibres in both the 20% EOCz- and trans-AT-treated mice.
Conclusion: Our data indicate that EOCz exerts significant wound healing activity, demonstrating its relevant therapeutic potential.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jep.2012.08.030 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Biological and metabolomics-guided isolation of tetrahydrofurofuran lignan from Croton spp. with antiproliferative activity against human melanoma cell line.
Fitoterapia
September 2024
Laboratory of Phytochemistry, Medicinal Chemistry, and Metabolomics. Chemistry Institute, Federal University of Alfenas (UNIFAL-MG), Alfenas, MG 37130-001, Brazil. Electronic address:
The Croton genus (Euphorbiaceae) is recognized as a promising source for identifying bioactive compounds with antiproliferative activity. However, knowledge on the chemical composition and activity of Croton floribundus, Croton echinocarpus, and Croton zehntneri is limited. Thus, this study aimed to investigate the antiproliferative activity of these species on cells derived from tumoral breast, lung, and melanoma cells, and primary fibroblasts derived from human skin.
View Article and Find Full Text PDFHigh Doses of Essential Oil of Induces Renal Tubular Damage.
Plants (Basel)
July 2021
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21.941-902, Brazil.
The essential oil of (EOCZ) and its major compounds are known to have several biological activities. However, some evidence shows potential toxic effects of high doses of EOCZ (>300 mg/kg) in amphibian and human kidneys. The aim of the present work was to investigate the effects on renal function of EOCZ at 300 mg/kg/day in healthy Swiss mice and a subclinical acute kidney injury (subAKI) animal model, which presents tubule-interstitial injury (TII).
View Article and Find Full Text PDFEssential Oil of Prevents Conduction Alterations Produced by Diabetes Mellitus on Vagus Nerve.
Plants (Basel)
April 2021
Laboratory of Electrophysiology, Superior Institute of Biomedical Science, State University of Ceará, Fortaleza CEP 60.740-000, Brazil.
Autonomic diabetic neuropathy (ADN) is a complication of diabetes mellitus (DM), to which there is no specific treatment. In this study, the efficacy of the essential oil of (EOCz) in preventing ADN was evaluated in the rat vagus nerve. For the two fastest conducting myelinated types of axons of the vagus nerve, the conduction velocities and rheobase decreased, whilst the duration of the components of the compound action potential of these fibers increased.
View Article and Find Full Text PDFAntinociceptive effect of triterpene acetyl aleuritolic acid isolated from Croton zehntneri in adult zebrafish (Danio rerio).
Biochem Biophys Res Commun
January 2021
State University of Ceará, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil; State University of Vale do Acaraú, Chemistry Course, Sobral, CE, Brazil; Department of Biological Chemistry, Regional University of Cariri, CE, Brazil. Electronic address:
Croton zehntneri is a plant known as canelinha de cunhã, prevalent in the northeast region of Brazil. Many constituents of the vegetable have already been studied, and their pharmacological properties have been proven, but this is the first study to analyze the antinociceptive effect in adult zebrafish (ZFa) of the triterpene acetyl aleuritolic acid (AAA) isolated from the stem bark. The animals (ZFa; n = 6/group) were treated intraperitoneally (ip; 20 μL) with AAA (0.
View Article and Find Full Text PDFAntispasmodic effects of the essential oil of , anethole, and estragole, on tracheal smooth muscle.
Heliyon
November 2020
Laboratory of Electrophysiology, Superior Institute of Biomedical Sciences, State University of Ceará, Fortaleza, Ceará, Brazil.
is a plant well adapted to the semi-arid climate of northeastern region of Brazil. The essential oil of (EOCz) has been described to have several pharmacologic properties, including effect on airflow resistance of respiratory system. For this reason, we investigated the hypothesis that EOCz and its major constituents, anethole and estragole, have antispasmodic activity on tracheal muscle.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!