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Genomic surveillance of multidrug-resistant organisms based on long-read sequencing.
Genome Med
November 2024
Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Article Synopsis
- Multidrug-resistant organisms (MDRO) are a major public health concern, and understanding their genomic characteristics is vital for monitoring and controlling infections.
- This study focuses on the use of long-read whole-genome sequencing (WGS) to analyze the molecular profiles of 356 MDRO isolates, including various bacteria such as Klebsiella pneumoniae and methicillin-resistant Staphylococcus aureus (MRSA).
- Results showed that long-read sequencing provides comparable molecular data to short-read sequencing, with high consistency in multi-locus sequence typing (wgMLST) profiles, though some differences were noted in Pseudomonas aeruginosa.
Characterization of quinolone resistance in Salmonella enterica serovar Typhimurium and its monophasic variants from food and patients in China.
J Glob Antimicrob Resist
December 2023
Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China. Electronic address:
Article Synopsis
- - The study investigated quinolone resistance in *Salmonella enterica serovar Typhimurium* and its variation *Salmonella enterica serovar 1,4,[5],12:i:-* from food and patients in China, assessing their susceptibility and genetic mutations.
- - Results showed high resistance rates to nalidixic acid, reduced susceptibility to ciprofloxacin and levofloxacin, and frequent mutations in the *gyrA* gene, along with several other resistance genes identified in the isolates.
- - The findings indicate a significant public health threat due to the clonal spread and horizontal gene transfer of quinolone resistance among these Salmonella strains in China.
Genetic Characterization of spp.: Whole Genome Sequencing-Based Approach for the Determination of Multiple Locus Variable Number Tandem Repeat Profiles.
Front Microbiol
November 2021
Bioinformatics Unit, Department of Infectious Diseases, National Institute of Health, Lisbon, Portugal.
Brucellosis is an important zoonosis that is emerging in some regions of the world, gaining increased relevance with the inclusion of the causing agent spp. in the class B bioterrorism group. Until now, multi-locus VNTR Analysis (MLVA) based on 16 loci has been considered as the gold standard for typing.
View Article and Find Full Text PDFIncreased Macrolide Resistance Rate of M3562 Correlated With Macrolide Usage and Genotype Shifting.
Front Cell Infect Microbiol
July 2021
Children's Hospital Affiliated to Zhengzhou University Henan Children's Hospital Zhengzhou Children's Hospital, Zhengzhou, China.
To characterize (MP) strains and to clarify the continuous high rates of macrolide resistance, 1,524 oropharyngeal swabs collected from children in Beijing Children's Hospital infected with MP during 2016-2019 were analyzed. Among the 1,524 samples, 1,386 harbored mutations associated with macrolide resistance; 1,049 samples were successfully classified into 11 genotypes using multiple locus variable-number tandem-repeat analysis (MLVA). The proportion of the predominant type, M4572, decreased from 84.
View Article and Find Full Text PDFDevelopment of a novel, high resolution melting analysis based genotyping method for Cryptosporidium parvum.
Eur J Protistol
June 2021
Department of Biological Sciences, Munster Technological University, Bishopstown, Cork, Ireland.
This study employed the post-real-time PCR application, high resolution melting (HRM) analysis, in order to differentiate between characterised clinical and reference Cryptosporidium parvum samples obtained from Cork University Hospital (Cork, Ireland) and the Cryptosporidium Reference Unit (Swansea, Wales). A sample set composed of 18 distinct C. parvum gp60-subtypes of the IIa gp60-subtype family (an allele family accounting for over 80% of all cryptosporidiosis cases in Ireland) was employed.
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