The role of mass spectrometry in the analysis of vitamin D compounds.

Mass Spectrom Rev

Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, USA.

Published: May 2013

AI Article Synopsis

  • Mass spectrometry is highlighted as the superior method for characterizing and quantifying vitamin D compounds, outperforming other methods like UV and bioassays in sensitivity and specificity.
  • The review begins with a brief introduction to the biochemistry of vitamin D compounds before discussing various techniques for their analysis.
  • The emphasis is placed on how mass spectrometry contributes significantly to the accurate measurement of vitamin D levels.

Article Abstract

This review highlights the superseding role of mass spectrometry in the structural characterization and quantitation of vitamin D compounds in comparison to other analytical methods (e.g., UV, bioassays) that lack the sensitivity and specificity of mass spectrometry. After a short introduction to the biochemistry of vitamin D compounds, an overview of the current techniques to characterize and quantitate vitamin D compounds is given with emphasis on the contribution of mass spectrometry.

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http://dx.doi.org/10.1002/mas.21353DOI Listing

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