AI Article Synopsis

  • The study explored a new nanoparticulate system using a terpolymer composed of starch, polymethacrylic acid, and polysorbate 80 to effectively load and release doxorubicin (Dox) in response to pH changes, targeting multidrug resistance in breast cancer cells.
  • Characterization techniques like FTIR, ITC, TEM, and DLS were employed to assess the properties of the Dox-loaded nanoparticles (Dox-NPs), which showed a high Dox loading efficiency of 99.9% and stable colloidal properties.
  • The Dox-NPs demonstrated significantly improved uptake and cytotoxic effects against human MDR1 breast cancer cells, with a 20-fold reduction in IC50

Article Abstract

This work investigated the capability of a new nanoparticulate system, based on terpolymer of starch, polymethacrylic acid and polysorbate 80, to load and release doxorubicin (Dox) as a function of pH and to evaluate the anticancer activity of Dox-loaded nanoparticles (Dox-NPs) to overcome multidrug resistance (MDR) in human breast cancer cells in vitro. The Dox-NPs were characterized by Fourier transform infrared spectroscopy (FTIR), isothermal titration calorimetry (ITC), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The cellular uptake and cytotoxicity of the Dox-loaded nanoparticles were investigated using fluorescence microscopy, flow cytometry, and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. The nanoparticles were able to load up to 49.7±0.3% of Dox with a high loading efficiency of 99.9±0.1%, while maintaining good colloidal stability. The nanoparticles released Dox at a higher rate at acidic pH attributable to weaker Dox-polymer molecular interactions evidenced by ITC. The Dox-NPs were taken up by the cancer cells in vitro and significantly enhanced the cytotoxicity of Dox against human MDR1 cells with up to a 20-fold decrease in the IC50 values. The results suggest that the new terpolymeric nanoparticles are a promising vehicle for the controlled delivery of Dox for treatment of drug resistant breast cancer.

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http://dx.doi.org/10.1016/j.ejpb.2012.09.001DOI Listing

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