AI Article Synopsis
- Immunohistochemistry is a technique that uses antibodies to pinpoint protein or antigen locations in complex tissues, including nonprotein antigens like BrdU.
- The process involves fixing, processing, and sectioning tissues before applying a primary antibody, ensuring tissue integrity remains intact.
- Methods for visualizing the antibodies include using reporter molecules detectable through light or fluorescent microscopy, with a focus on testing protein localization in testis sections.
Article Abstract
Immunohistochemistry is an important technique that uses specific antibodies to determine the cellular localization of proteins/antigens in highly complex organs and tissues. While most immunohistochemistry experiments target protein epitopes, nonprotein antigens including BrdU may also be detected. Briefly, tissues are fixed, processed, sectioned, and then probed by a primary antibody while preserving the integrity of the tissue and cellular morphology. There are various methods available for visualization of the bound primary antibody that involve a reporter molecule which can be detected using light or fluorescent microscopy. Here we describe a basic immunohistochemistry protocol for identifying protein localization in testis sections using protein-specific antibodies.
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| http://dx.doi.org/10.1007/978-1-62703-038-0_28 | DOI Listing |
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