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Single-shot quantitative dispersion phase microscopy. | LitMetric

Single-shot quantitative dispersion phase microscopy.

Appl Phys Lett

George R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

Published: August 2012

We present an imaging modality capable of providing high-speed optical dispersion measurements of live cells. The technique permits wide-field measurement of the optical phase shifts introduced by a sample for multiple discrete wavelengths in a single image capture. Utilizing spatial modulation and the wavelength dependence of the interference-fringe spacing, average refractive index as a function of wavelength is obtained, yielding optical dispersion measurements of the sample under observation. Because of its simple and low-cost design, the technique can be readily integrated into a standard microscope to collect additional diagnostic information about biological cells.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3436913PMC
http://dx.doi.org/10.1063/1.4745785DOI Listing

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