AI Article Synopsis

  • Epitope-based vaccination could enhance immunity against Japanese encephalitis virus (JEV), and this study evaluates the immune response to a recombinant vaccine (rMVA-mep) containing multiple JEV epitopes.
  • Researchers cloned the multi-epitope gene into a vector and immunized mice with various doses, finding that the highest immune response occurred at a dosage of 107 TCID50.
  • The rMVA-mep vaccine stimulated strong immune responses, promoting both Th1 and Th2 responses, and provided effective protection against lethal JEV, suggesting its potential as a viable vaccine candidate.

Article Abstract

Epitope-based vaccination might play an important role in the protective immunity against Japanese encephalitis virus (JEV) infection. The purpose of the study is to evaluate the immune characteristics of recombinant MVA carrying multi-epitope gene of JEV (rMVA-mep). The synthetic gene containing critical epitopes (B-cell, CTL and Th) of JEV was cloned into the eukaryotic expression vector pGEM-K1L, and the rMVA-mep was prepared. BALB/c mice were immunized with different dosages of purified rMVA-mep and the immune responses were determined in the form of protective response against JEV, antibodies titers (IgG1 and IgG2a), spleen cell lymphocyte proliferation, and the levels of interferon-γ and interleukin-4 cytokines. The results showed that live rMVA-mep elicited strongly immune responses in dose-dependent manner, and the highest level of immune responses was observed from the groups immunized with 107 TCID50 rMVA-mep among the experimental three concentrations. There were almost no difference of cytokines and neutralizing antibody titers among 107 TCID50 rMVA-mep, recombinant ED3 and inactivated JEV vaccine. It was noteworthy that rMVA-mep vaccination potentiates the Th1 and Th2-type immune responses in dose-dependent manner, and was sufficient to protect the mice survival against lethal JEV challenge. These findings demonstrated that rMVA-mep can produce adequate humoral and cellular immune responses, and protection in mice, which suggested that rMVA-mep might be an attractive candidate vaccine for preventing JEV infection.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3511265PMC
http://dx.doi.org/10.1186/1743-422X-9-204DOI Listing

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