Background: Infections by A. calcoaceticus-A. baumannii (ACB) complex isolates represent a serious threat for wounded and burn patients. Three international multidrug-resistant (MDR) clones (EU clone I-III) are responsible for a large proportion of nosocomial infections with A. baumannii but other emerging strains with high epidemic potential also occur.
Methodology/principal Findings: We automatized a Multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) protocol and used it to investigate the genetic diversity of 136 ACB isolates from four military hospitals and one childrens hospital. Acinetobacter sp other than baumannii isolates represented 22.6% (31/137) with a majority being A. pittii. The genotyping protocol designed for A.baumannii was also efficient to cluster A. pittii isolates. Fifty-five percent of A. baumannii isolates belonged to the two international clones I and II, and we identified new clones which members were found in the different hospitals. Analysis of two CRISPR-cas systems helped define two clonal complexes and provided phylogenetic information to help trace back their emergence.
Conclusions/significance: The increasing occurrence of A. baumannii infections in the hospital calls for measures to rapidly characterize the isolates and identify emerging clones. The automatized MLVA protocol can be the instrument for such surveys. In addition, the investigation of CRISPR/cas systems may give important keys to understand the evolution of some highly successful clonal complexes.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3440325 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0044597 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Foeniculum vulgare Miller bracts, revalorization of a local food waste.
Sci Rep
December 2024
Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, Fisciano, 84084, Salerno, Italy.
This research aims at the valorization of fennel by-products from the Campania region (Southern Italy). A phytochemical characterization of the hydroalcoholic extracts (HEs) and of the essential oils (EOs) from edible and non-edible parts (waste) of Foeniculum vulgare Mill. was carried out using HRESIMS and GC-MS.
View Article and Find Full Text PDFSilent circulation of plasmid-borne tet(X6) and bla genes in a community-acquired Acinetobacter baumannii strain.
Drug Resist Updat
December 2024
Department of Clinical Laboratory, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China; Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province, Hangzhou, China. Electronic address:
To characterize the genomic features of a community-acquired Acinetobacter baumannii strain, co-carrying tet(X6) and bla genes, but was susceptible to tigecycline and carbapenems. The tet(X6) and bla genes were found on a 149,518 bp non-conjugative plasmid. The bla gene was silent, due to the presence of an intact ISAba3-like element upstream, which rendered the strain susceptible to carbapenems.
View Article and Find Full Text PDFRapid and precise treatment selection for antimicrobial-resistant infection enabled by a nano-dilution SlipChip.
Biosens Bioelectron
December 2024
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China; Zhengzhou Industrial Technology Research Institute of Shanghai Jiao Tong University, Zhengzhou, 450016, China. Electronic address:
Antimicrobial resistance (AMR) has become an increasingly severe threat to global health, and AMR-associated infection is one of the leading causes of death around the world. Due to the long turnaround time and the limited flexibility and availability of current antimicrobial susceptibility testing (AST) methods, a large portion of patients with bacterial infections are still treated empirically, increasing the risk of mistreatment. To address the demand for precision treatment of bacterial infections, we developed a nano-dilution SlipChip (nd-SlipChip)-based systematic evaluation method, which facilitates rapid, logic feedback for the assessment of antibiotics, antibiotic combinations, and phage therapy.
View Article and Find Full Text PDFCarbapenem-induced β-lactamase-isoform expression trends in Acinetobacter baumannii.
Sci Rep
December 2024
Department of Pharmaceutical Sciences, Northeast Ohio Medical University, Rootstown, OH, USA.
Carbapenem-resistant Acinetobacter baumannii (CRAb) is an urgent bacterial threat to public health, with only a few treatment options and a > 50% fatality rate. Although several resistance mechanisms are understood, it is still impossible to predict which mutations are most likely to occur. Here, we demonstrate that independent samples of Ab, exposed to different carbapenems with escalating concentrations, show concentration- and carbapenem-dependent trends in β-lactamase-isoform expression.
View Article and Find Full Text PDFBiodegradation of plasticizers by novel strains of bacteria isolated from plastic waste near Juhu Beach, Mumbai, India.
Sci Rep
December 2024
Computer Aided Drug Designing and Molecular Modeling Lab, Department of Bioinformatics, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India.
Phthalic acid esters are pivotal plasticizers in various applications, including cosmetics, packaging materials, and medical devices. They have garnered significant attention from the scientific community due to their persistence in ecosystems. The multifaceted aspects of PAEs, encompassing leaching, transformation, and toxicity, underscore their prominence as primary components of anthropogenic waste.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!