Aim: To detect the concentration of soluble FcγRIIb in blood sera of SLE patients and healthy controls, then to obtain recombinant human soluble FcγRIIb protein (husFcγRIIb) in Escherichia coli (E.coli) and examine its binding capability with immune complexes (IC) and its effect on IgM secretion by B cells.
Methods: The concentration of husFcγRIIb in blood sera of SLE patients and healthy controls was detected by ELISA. E.coli BL21, containing pET-sFcγRIIb, was stimulated by IPTG to induce husFcγRIIb expression, and then husFcγRIIb protein was purified by Ni-NTA agarose bead system. The IC-binding ability of husFcγRIIb was detected by ELISA. Furthermore, B cells were sorted by immune magnetic bead from human peripheral blood and challenged by different stimulators under the condition of husFcγRIIb or not for 10 d, then the concentration of IgM in supernatant was detected by ELISA.
Results: The concentration of husFcγRIIb in the serum of SLE patients was lower than that in the controls (P<0.05). The recombinant husFcγRIIb protein was successfully expressed and purified with M(r); being 41 500. It could combine with IC and the absorption became higher with the increasing concentration of IC. After 10-day stimulation on the B cells, the titer of IgM between SPA and SPA+husFcγRIIb groups was not significantly different (P>0.05), and the titer was higher in SPA+anti-IgM group than SPA group (P<0.01). Interestingly, the titer of IgM in SPA+husFcγRIIb+anti-IgM group was lower than SPA+anti-IgM group (P<0.01), SPA group (P<0.01) and SPA+husFcγRIIb group (P<0.01).
Conclusion: The concentration of husFcγRIIb in the serum of SLE patients is lower than that in the healthy controls. The recombinant husFcγRIIb protein can combine with IC and inhibit IgM antibody secretion by B cells.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Cardiovascular disease risk in Korean patients with systemic lupus erythematosus compared to diabetes mellitus and the general population.
Sci Rep
January 2025
Department of Rheumatology, Hanyang University Hospital for Rheumatic Diseases, Seoul, 04763, Republic of Korea.
To evaluate the incidence and risk of cardiovascular disease (CVD) among Korean patients with systemic lupus erythematosus (SLE) comparing them to diabetes patients and the general population. This nationwide cohort study focused on incident SLE patients aged over 40 years, matched with diabetes patients and the general population (1:4:4 ratio). CVD was defined as ischaemic heart disease, ischaemic stroke, and cardiac arrest.
View Article and Find Full Text PDFTrends in work participation among patients with inflammatory rheumatic musculoskeletal diseases (iRMDs): Data from the German National Database (2010-2022).
RMD Open
January 2025
Epidemiology and Health Services Research, German Rheumatology Research Center, Berlin, Germany.
Objective: To analyse work participation among patients with inflammatory rheumatic musculoskeletal diseases (iRMDs), namely rheumatoid arthritis (RA), axial spondyloarthritis (axSpA), psoriatic arthritis (PsA), systemic lupus erythematosus (SLE), systemic sclerosis (SSc) and ANCA-associated vasculitis (AAV).
Methods: A cross-sectional sample of 16 421 patients from the National Database of the German Collaborative Arthritis Centers, aged <65 years were analysed. For each diagnosis, yearly rates of absenteeism, employment and disability pensions were analysed from 2010 to 2022.
Neuropsychiatric manifestations in systemic lupus erythematosus and Sjogren's disease.
Autoimmun Rev
January 2025
Department of Orthopedics, Rheumatology and Traumatology-School of Medical Sciences, University of Campinas, Brazil; Autoimmunity Lab, School of Medical Sciences, University of Campinas, Brazil. Electronic address:
Introduction: Autoimmune diseases often present in a systemic manner, affecting various organs and tissues. Involvement of the central and peripheral nervous system is not uncommon in these conditions and is associated with high morbidity and mortality. Therefore, early recognition of the neuropsychiatric manifestations associated with rheumatologic diseases is essential for the introduction of appropriate therapies with the objective of providing a better quality of life for individuals.
View Article and Find Full Text PDFSenear-Usher Syndrome or Coexistence of SLE with Pemphigus Vulgaris-A Case Report with Literature Review.
J Clin Med
January 2025
Department of Dermatology and Venereology, Medical University of Lodz, pl. Hallera 1, 90-647 Lodz, Poland.
Senear-Usher syndrome, or pemphigus erythematosus (PE), is a rare autoimmune disorder characterized by the coexistence of features from both lupus erythematosus (LE) and pemphigus foliaceus (PF). We describe a 41-year-old patient initially diagnosed with cutaneous and then systemic lupus erythematosus (SLE), who after a few years developed new skin lesions: erythematous and erosive eruptions partially covered by crusts located on the trunk and flaccid blisters on the extremities. Direct immunofluorescence of perilesional skin revealed deposits of IgG in the intercellular space of the epidermis and granular deposits of C3 at the dermo-epidermal junction.
View Article and Find Full Text PDFM1 and M2 Macrophage Polarization Correlates with Activity and Chronicity Indices in Lupus Nephritis.
Life (Basel)
January 2025
Department of Anatomical Pathology, Faculty of Medicine Vajira Hospital, Navamindradhiraj University, 681 Samsen Road, Dusit, Bangkok 10300, Thailand.
Lupus nephritis (LN) is a severe manifestation of systemic lupus erythematosus (SLE), characterized by inflammation and immune dysregulation in the kidneys. The role of macrophage polarization in LN progression remains underexplored. This study examined the association between tubulointerstitial M1/M2 macrophage subpopulations and LN indices of activity and chronicity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!