The release of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from human erythrocyte membranes lysed by hemolysin of Prevotella oris.

Anaerobe

Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.

Published: October 2012

AI Article Synopsis

  • A 38-kDa protein was released from erythrocyte membranes when exposed to Prevotella oris hemolysin, but not during hypotonic hemolysis.
  • The protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH) through N-terminal amino acid sequencing.
  • The study explores the connection between GAPDH and the process of hemolysis.

Article Abstract

We found that a 38-kDa protein was released from erythrocyte membranes lysed by hemolysin of Prevotella oris, although hypotonic hemolysis did not show such a phenomenon. The 38-kDa protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by N-terminal amino acid sequencing. This study discusses the relationship between GAPDH and hemolysis.

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http://dx.doi.org/10.1016/j.anaerobe.2012.08.008DOI Listing

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