AI Article Synopsis
- Recent interest in studying inactive conformations of kinases has led to the development of new biochemical tools.
- A fluorescent probe has been created that specifically binds to a certain inactive form of protein kinases.
- This probe enables researchers to examine the thermodynamics and kinetics of how ligands bind to these kinases.
Article Abstract
Recent interest in inactive kinase conformations has generated the need to develop new biochemical tools to study them. Here, we describe the use of a fluorescent probe that selectively and potently binds to a specific inactive conformation of protein kinases. This allows for the thermodynamics and kinetics of ligand binding to be determined.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5228460 | PMC |
| http://dx.doi.org/10.1007/978-1-62703-008-3_12 | DOI Listing |
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