AI Article Synopsis
- The study investigates the expression of the α(5) subunit of extrasynaptic GABA(A) receptors in rats with induced insular epilepsy, using various control groups for comparison.
- Results showed no significant difference in the number of hippocampal neurons across groups, but both mRNA levels and protein expression of the α(5) subunit were significantly lower in the kindled group compared to control and sham-operated groups at two different time points.
- The findings suggest that decreased expression of the α(5) subunit in the hippocampus may be linked to the development and progression of insular epilepsy, indicating its potential role in this condition.
Article Abstract
Objective: To explore the expression of α(5) subunit of extrasynaptic GABA(A) receptor in insular electrical kindled rats and analyze its significance.
Methods: A total of 96 male Sprague-Dawley rats were divided randomly into kindled, sham-operated and control groups (n = 32). And each group was further divided into 2 sub-groups at different time points. Kindled group: insular electrical kindled via chronic electrical stimulation; Sham-operated group: electrode implantation without electrical stimulation;
Control Group: no operation at all. The number of hippocampal neurons was detected by Nissl staining; the mRNA of α(5) subunit of hippocampus by quantitative-polymerase chain reaction (q-PCR); the expression of α(5) subunit of hippocampus by immunohistochemistry.
Results: No significant difference existed in the number of hippocampal neurons between epileptic and normal rats (P > 0.05). The mRNA levels of α(5) subunit of hippocampus in the sham-operated and control groups were much higher than that of the kindled group at Day 1 and 7 post-sacrificing (P < 0.01). And the hippocampal expressions of α(5) subunit were much more in the sham-operated and control groups than that of the kindled group at Day 1 and 7 post-sacrificing (P < 0.01).
Conclusion: The hippocampal expression of α(5) subunit decreases markedly in insular epilepsy. And α(5) subunit may play an important role in the occurrence and development of insular epilepsy.
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