[Promoting effects of CYR61 on proliferation, migration and tube formation of choroid-retinal endothelial cells].

Objective: To observe the effects of CYR61 (cysteine-rich 61; CCN1) on the proliferation, migration and tube formation of choroid-retinal endothelial cells (RF-6A cell line).

Methods: Experimental study. RF-6A cells were cultured and treated with CYR61 at different concentrations. Effects of CYR61 on cell proliferation, migration and angiogenesis were observed by MTT assay, transwell assay and tube formation assay.

Results: When different concentrations of CYR61 (0, 5, 10, 100 and 500 µg/L) were used to treat RF-6A cells for 72 h, A(490) nm value of the MTT assay was changed dose-dependently (0.511, 0.522, 0.532, 0.597, 0.765 and 0.818), and the difference between different dosage groups was statistically significant (F = 318.828, P < 0.05). When RF-6A cells were treated with 400 µg/L CYR61 for different time periods (0, 12, 24, 48 and 72 h), A(490) nm value increased with the extension of treatment time (0.533, 0.598, 0.643, 0.695 and 0.756), and the difference was statistically significant (F = 42.910, P < 0.05). In transwell assay, migrated cells in cells treated with different concentrations of CYR61 (40, 200, 400 µg/L and 400 µg/L + 25 mg/Lanti-CYR61 antibody), 80 µg/L VEGF, and negative control groups were 66.83 ± 3.87, 77.83 ± 4.26, 96.83 ± 3.49, 70.67 ± 3.83, 98.33 ± 3.14 and 62.00 ± 7.62 per high-power field, respectively. RF-6A cell migration capacity increased with increased concentration of CYR61 (F = 46.987, P < 0.05). In tube formation assay, numbers of tube in different concentrations of CYR61 (40, 200, 400 µg/L, 400 µg/L + 25 mg/L anti-CYR61 antibody), 80 µg/L VEGF and negative control groups were 34.33 ± 2.50, 60.67 ± 3.72, 88.17 ± 2.93, 51.17 ± 2.14, 90.83 ± 3.49 and 31.83 ± 3.31 per field. RF-6A cell tube formation capacity increased with increased concentration of CYR61 (F = 355.224, P < 0.05). There were equal effects between 400 µg/L CYR61 and 80 µg/L VEGF. Anti-CYR61 antibody could inhibit cell migration and tube formation promoted by CYR61.

Conclusions: CYR61 can promote proliferation, migration and tube formation of choroid-retinal endothelial cells in vitro. CYR61 are likely to be involved in the pathogenesis of retinal neovascularization.