L1 is a major granulocyte and monocyte protein with a Mr of 36.5 kDa. It is found mainly in the cytosol of these cells. Purified L1 is shown, on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), to contain three subunits. In this study, 6 mol/l concentration of urea was found to be sufficient for disassembly of the polypeptides, and urea-containing preparative isoelectric focusing gel was used for separation of high quantities of the subunits. The pI of the eluted subunits were 5.8, 6.1 and 7.1. When tested on 2D-PAGE, the isolated subunits were found at their typical locations. Polyclonal rabbit antibodies were produced against the subunits, and the antisera were, on dot-blot, found to react with the different subunits as well as the purified L1.
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