Previous studies demonstrated the importance of substrate stiffness and topography on the phenotype of many different cell types including fibroblasts. Yet the interaction of these two physical parameters remains insufficiently characterized, in particular for cardiac fibroblasts. Most studies focusing on contact guidance use rigid patterned substrates. It is not known how the ability of cardiac fibroblasts to follow grooves and ridges changes as the substrate stiffness is decreased to match the range of stiffness found in native heart tissues. This report demonstrates a significant interactive effect of substrate stiffness and topography on cardiac fibroblast elongation and orientation using polyacrylamide substrates of different stiffness and topography.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/mabi.201200042 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Predicting liver fibrosis in Gaucher disease: Investigation of contributors and development of a clinically applicable Gaucher liver fibrosis score.
Mol Genet Metab
January 2025
Medical Genetics Service, HCPA, UFRGS, Porto Alegre, RS, Brazil; Graduate Program in Genetics and Molecular Biology, UFRGS, Porto Alegre, RS, Brazil; InRaras (National Institute of Science and Technology on Rare Diseases), Brazil.
Gaucher disease (GD) is a rare genetic disorder with multi-system involvement. Liver fibrosis is a long-term complication of GD, potentially leading to cirrhosis, end-stage liver disease, and hepatocellular carcinoma. There are currently no validated clinical tools for the monitoring of liver fibrosis in patients with GD.
View Article and Find Full Text PDFCellulose-mediated mechanical property tuning in small intestinal submucosal matrix to enhance stem cell osteogenic differentiation.
Int J Biol Macromol
January 2025
School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China; GWDC Kunshan Company, Kunshan 215337, China; Jingkun Chemistry Company, Kunshan 215337, China. Electronic address:
Natural extracellular matrices (ECM) provide a more accurate simulation of the cellular growth environment, making them excellent substrate materials for in vitro cell culture. The porcine small intestinal submucosa (SIS) is one of the most widely used natural ECM that display superior bioactivity. However, decellularization operations often result in fiber breakage and failure to recover mechanical strength in the SIS.
View Article and Find Full Text PDFTissue-Adhesive and Stiffness-Adaptive Neural Electrodes Fabricated Through Laser-Based Direct Patterning.
Small Methods
January 2025
Department of Electrical and Computer Engineering, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
Recently, implantable devices for treating peripheral nerve disorders have demonstrated significant potential as neuroprosthetics for diagnostics and electrical stimulation. However, the mechanical mismatch between these devices and nerves frequently results in tissue damage and performance degradation. Although advances are made in stretchable electrodes, challenges, including complex patterning techniques and unstable performance, persist.
View Article and Find Full Text PDFChondrocytes are commonly applied in regenerative medicine and tissue engineering. Thus, the discovery of optimal culture conditions to obtain cells with good properties and behavior for transplantation is important. In addition to biochemical cues, physical and biomechanical changes can affect the proliferation and protein expression of chondrocytes.
View Article and Find Full Text PDFMesenchymal Stem Cell Plasticity: What Role Do Culture Conditions and Substrates Play in Shaping Biomechanical Signatures?
Bioengineering (Basel)
December 2024
Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, 72076 Tübingen, Germany.
Cell functionality, driven by remarkable plasticity, is strongly influenced by mechanical forces that regulate mesenchymal stem cell (MSC) fate. This study explores the biomechanical properties of jaw periosteal cells (JPCs) and induced mesenchymal stem cells (iMSCs) under different culture conditions. We cultured both JPCs and iMSCs (n = 3) under normoxic and hypoxic environments, with and without osteogenic differentiation, and on laminin- or gelatin-coated substrates.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!