Purpose: This study was designed to determine the tumorigenicity of the AS30D HCC cell line following orthotopic injection into rat liver and preliminarily characterize the tumor model by both magnetic resonance imaging (MRI) and ultrasound (US) as well as histopathology and immunohistochemistry.
Materials: AS30D cell line in vitro proliferation was assessed by using MTT assay. Female rats (N = 5) underwent injection of the AS30D cell line into one site in the liver. Rats subsequently underwent MR imaging at days 7 and 14 to assess tumor establishment and volume. One rat underwent US of the liver at day 7. Rats were euthanized at day 7 or 14 and livers were subjected to gross, histopathologic (H&E), and immunohistochemical (CD31) analysis to assess for tumor growth and neovascularization.
Results: AS30D cell line demonstrated an in vitro doubling time of 33.2 ± 5.3 h. MR imaging demonstrated hyperintense T2-weighted and hypointense T1-weighted lesions with tumor induction in five of five and three of three sites at days 7 and 14, respectively. The mean (SD) tumor volume was 126.1 ± 36.2 mm(3) at day 7 (N = 5). US of the liver demonstrated a well-circumscribed, hypoechoic mass and comparison of tumor dimensions agreed well with MRI. Analysis of H&E- and CD31-stained sections demonstrated moderate-high grade epithelial tumors with minimal tumor necrosis and evidence of diffuse intratumoral and peritumoral neovascularization by day 7.
Conclusions: AS30D HCC cell line is tumorigenic following orthotopic injection into rat liver and can be used to generate an early vascularizing, slower-growing rat HCC tumor model.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3904804 | PMC |
| http://dx.doi.org/10.1007/s00270-012-0466-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Modulators of mitochondrial ATP-sensitive potassium channel affect cytotoxicity of heavy metals: Action on isolated rat liver mitochondria and AS-30D ascites hepatoma cells.
Ecotoxicol Environ Saf
May 2023
I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry RAS, Thorez av. 44, 194223, St.-Petersburg, Russia. Electronic address:
Heavy metals are ubiquitous environmental pollutants that are extremely dangerous for public health, but the molecular mechanisms of their cytotoxic action are still not fully understood. In the present work, the possible contribution of the mitochondrial ATP-sensitive potassium channel (mK(ATP)), which is usually considered protective for the cell, to hepatotoxicity caused by heavy metals was investigated using polarography and swelling techniques as well as flow cytometry. Using isolated liver mitochondria from adult male Wistar rats and various potassium media containing or not containing penetrating anions (KNO, KSCN, KAcet, KCl), we studied the effect of mK(ATP) modulators, namely its blockers (5-hydroxydecanoate, glibenclamide, ATP, ADP) and activators (diazoxide, malonate), on respiration and/or membrane permeability in the presence of hepatotoxins such as Cd, Hg, and Cu.
View Article and Find Full Text PDFQuantitative Expression of Key Cancer Markers in the AS-30D Hepatocarcinoma Model.
Front Oncol
October 2021
Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Article Synopsis
- Hepatocellular carcinoma (HCC) has a high mortality rate and is often diagnosed late, highlighting the need for effective biomarkers in cancer research.
- The study focuses on quantifying the mRNA levels of key signaling molecules involved in tumorigenesis, particularly those related to cancer hallmarks and epithelial-mesenchymal transition (EMT), using a murine transplantable hepatocarcinoma model (AS-30D).
- Results showed elevated mRNA levels of master regulators of EMT and a mesenchymal signature in AS-30D cells, indicating they are a useful and cost-effective model for studying HCC-related gene expression changes.
Regulatory role of acetylation on enzyme activity and fluxes of energy metabolism pathways.
Biochim Biophys Acta Gen Subj
December 2021
Departamento de Bioquímica, Instituto Nacional de Cardiología, Mexico City 14080, Mexico. Electronic address:
Background: Most of the enzymes involved in the central carbon metabolism are acetylated in Lys residues. It has been claimed that this covalent modification represents a novel regulatory mechanism by which both enzyme/transporter activities and pathway fluxes can be modulated.
Methods: To establish which enzymes are regulated by acetylation, a systematic experimental analysis of activities and acetylation profile for several energy metabolism enzymes and pathway fluxes was undertaken in cells and mitochondria.
Activation of ALDH1A1 by omeprazole reduces cell oxidative stress damage.
FEBS J
July 2021
Departamento de Bioquímica, Instituto Nacional de Cardiología 'Ignacio Chávez', Ciudad de México, México.
Under physiological conditions, cells produce low basal levels of reactive oxygen species (ROS); however, in pathologic conditions ROS production increases dramatically, generating high concentrations of toxic unsaturated aldehydes. Aldehyde dehydrogenases (ALDHs) are responsible for detoxification of these aldehydes protecting the cell. Due to the physiological relevance of these enzymes, it is important to design strategies to modulate their activity.
View Article and Find Full Text PDFKinetic modeling of glucose central metabolism in hepatocytes and hepatoma cells.
Biochim Biophys Acta Gen Subj
November 2020
Departamento de Bioquímica, Instituto Nacional de Cardiología, Mexico City 14080, Mexico. Electronic address:
Background: Kinetic modeling and control analysis of a metabolic pathway may identify the steps with the highest control in tumor cells, and low control in normal cells, which can be proposed as the best therapeutic targets.
Methods: Enzyme kinetic characterization, pathway kinetic modeling and control analysis of the glucose central metabolism were carried out in rat (hepatoma AS-30D) and human (cervix HeLa) cancer cells and normal rat hepatocytes.
Results: The glycogen metabolism enzymes in AS-30D, HeLa cells and hepatocytes showed similar kinetic properties, except for higher AS-30D glycogen phosphorylase (GP) sensitivity to AMP.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!