Citrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replication cycle of these viruses, the subcellular localization of the viral coat proteins (CP) was studied. Immunoblot analysis of fractionated extracts derived from natural and experimental infected hosts indicated that the CP of CPsV occurs in the soluble cytoplasmic fraction. The cytoplasmic localization of this protein was confirmed by confocal microscopy of fluorescent protein (FP)-tagged CP following its expression in either CPsV-infected and healthy Citrus sinensis plants or in Nicotiana benthamiana plants. The same localization was observed for FP-tagged CP of MiLBVV. The CPs of CPsV and MiLBBV can undergo homologous and heterologous interactions as revealed by fluorescent lifetime imaging microscopy and co-immunoprecipitation analysis. A putative leucine zipper motif that is conserved among ophiovirus CP sequences may account for these interactions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.virusres.2012.08.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of TaqMan-Based Real-Time qPCR Method for Accurate Detection and Quantification of Citrus Psorosis Virus and Cytoplasmic-Type Citrus Leprosis Virus in Saplings.
Plant Pathol J
December 2024
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea.
In 2022, citrus fruits were the second most widely produced fruit globally, highlighting their significant role in the fruit industry. However, due to their clonal propagation, these fruits are highly susceptible to viral infections, posing challenges for growers. In response to the booming nursery market, the Korean plant quarantine station reported over 80 million sapling stocks, with 15% being discarded after rigorous inspection due to contamination or disease.
View Article and Find Full Text PDFFirst Report of Citrus Leaf Blotch Virus on Nagami Kumquat in Turkey.
Plant Dis
September 2024
UC Davis, Plant Pathology, Davis, California, United States;
Article Synopsis
- - Kumquat orchards have been growing in Turkey, and a survey in May 2022 aimed to identify potential citrus virus diseases, particularly focusing on symptoms of bud union disorder found in some trees.
- - Testing for common citrus viruses, such as CLBV, showed that symptomatic trees tested positive for the virus, while symptomless trees did not, indicating a potential link between CLBV and the observed disorder.
- - The study used RT-PCR techniques to confirm the presence of CLBV by amplifying specific genes, with the sequences showing high similarity to known CLBV strains in genetic databases.
Citrus psorosis virus 24K protein inhibits the processing of miRNA precursors by interacting with components of the biogenesis machinery.
Microbiol Spectr
July 2024
Instituto de Biotecnología y Biología Molecular, CCT-La Plata, CONICET-UNLP, La Plata, Buenos Aires, Argentina.
Sweet orange () is one of the most important fruit crops worldwide. Virus infections in this crop can interfere with cellular processes, causing dramatic economic losses. By performing RT-qPCR analyses, we demonstrated that citrus psorosis virus (CPsV)-infected orange plants exhibited higher levels of unprocessed microRNA (miRNA) precursors than healthy plants.
View Article and Find Full Text PDFDevelopment of TaqMan RT-qPCR for the detection of regulated citrus viruses and viroids in Aotearoa New Zealand.
J Virol Methods
June 2024
Plant Health and Environment Laboratory, Ministry for Primary Industries, 231 Morrin Road, St. Johns, Auckland 1072, New Zealand. Electronic address:
The major citrus species include several economically important fruits, such as orange, mandarin, lemon, limes, grapefruit and pomelos. Since the 1980 s, total production and consumption of citrus has grown strongly with the current annual worldwide production at over 105 million tonnes. New Zealand's citrus exports, for instance, had an estimated worth of NZ$ 11.
View Article and Find Full Text PDFFirst Report of citrus virus A infecting citrus (Citrus reticulata Blanco) in China.
Plant Dis
December 2022
Chinese Academy of Agricultural Sciences Citrus Research Institute, 117459, Xiema, Beibei, Chongqing, China, 400712;
Citrus is one of the most popular fruit crops in the world. Citrus virus A (CiVA, species Coguvirus eburi, genus Coguvirus) is a newly identified virus (Navarro et al. 2018) with two negative-sense single-stranded RNAs (RNA1 and RNA2).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!