AI Article Synopsis
- Affinity determination is important for characterizing antibodies, and this text presents a method using flow cytometry for this purpose.* -
- The technique utilizes the strong bond between biotin and streptavidin for easy and efficient labeling of antibodies, with various labeling methods discussed.* -
- The document provides a detailed procedure for determining the dissociation constant (K[D]) using flow cytometry, outlining both chemical and enzymatic biotinylation techniques.*
Article Abstract
Affinity determination is a crucial step of an antibody characterization. Here, we describe a method for antibody affinity determination by flow cytometry, relying on the unique affinity of biotin for streptavidin for easy and efficient antibody labeling. Several labeling approaches are described and discussed in this chapter, including chemical and enzymatic (in vivo and in vitro) biotinylation. Finally, a procedure for K (D) determination by flow cytometry is precisely described.
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| http://dx.doi.org/10.1007/978-1-61779-974-7_25 | DOI Listing |
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