This work describes the evaluation of three ceramic materials as potential osteogenic substrate for bone tissue engineering. The capacity of adult human mesenchymal stem cells cultured under experimental conditions known to adhere, proliferate and differentiate into osteoblasts was studied. Two types of culture medium: growth medium and osteogenic medium were evaluated. The materials were pure α-tricalcium phosphate and also αTCP doped with either 1.5 or 3 wt% of dicalcium silicate. The results showed that the hMSCs cultured adhered, spread, proliferated and produced mineralized extracellular matrix on all the ceramics studied. They showed an osteoblastic phenotype, especially in the αTCP doped with 1.5 wt% C(2)S, indicating osteoblastic differentiation as a result of the increased concentration of silicon in solid solution in TCP. Ceramics evaluated in this work are bioactive, cytocompatible and capable of promoting the differentiation of hMSCs into osteoblast.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10856-012-4742-z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The transmembrane protein TMEM196 controls cell proliferation and determines the floor plate cell lineage.
Dev Growth Differ
January 2025
Division of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
The neural tube, the embryonic precursor to the vertebrate central nervous system, comprises distinct progenitor and neuronal domains, each with specific proliferation programs. In this study, we identified TMEM196, a novel transmembrane protein that plays a crucial role in regulating cell proliferation in the floor plate in chick embryos. TMEM196 is expressed in the floor plate, and its overexpression leads to reduced cell proliferation without affecting the pattern formation of the neural tube.
View Article and Find Full Text PDFBackground/aims: Bruise is the extravasation of blood that may be mild or severe. Bone marrow mesenchymal stem cells (BM-MSCs) are one of the most promising cells used in regenerative medicine for treating many disorders. We aimed to evaluate the efficiency of BM-MSCs in treating cutaneous bruises.
View Article and Find Full Text PDFHuman epicardial organoids from pluripotent stem cells resemble fetal stage with potential cardiomyocyte- transdifferentiation.
Cell Biosci
January 2025
Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China.
Epicardium, the most outer mesothelium, exerts crucial functions in fetal heart development and adult heart regeneration. Here we use a three-step manipulation of WNT signalling entwined with BMP and RA signalling for generating a self-organized epicardial organoid that highly express with epicardium makers WT1 and TCF21 from human embryonic stem cells. After 8-days treatment of TGF-beta following by bFGF, cells enter into epithelium-mesenchymal transition and give rise to smooth muscle cells.
View Article and Find Full Text PDFBCL-2 overexpression exosomes promote the proliferation and migration of mesenchymal stem cells in hypoxic environment for skin injury in rats.
J Biol Eng
January 2025
Department of Traumatic Clinic, Shanghai East Hospital of Tongji University, Shanghai, 200120, China.
Objective: The direction of this study was to detect and analyze the specific mechanism of anti-apoptosis in mesenchymal stem cells (MSCs) cells caused by high expression of BCL2.
Methods: Bioinformatics was completed in Link omics. GO analysis and KEGG analysis were carried out, and the grope tool of Link omics database was used to evaluate PPI information and other core path analysis information.
B-doped nano-hydroxyapatite facilitates proliferation and differentiation of osteoblasts.
J Orthop Surg Res
January 2025
Xuzhou Medical University Affiliated Stomatology Hospital, Xuzhou, 221002, Jiangsu Province, China.
Purpose: We aimed to explore the mechanism by which Boron-doped nano-hydroxyapatite (B-nHAp) facilitates the proliferation and differentiation of osteoblasts through controlled release of B.
Methods: B-nHAp characterization was accomplished by means of X-ray diffraction, scanning electron microscopy, inductively coupled plasma mass spectrometry, and transmission electron microscopy. Human bone marrow mesenchymal stem cells (hBMSCs) were subjected to flow cytometry, alizarin red S staining, and cell counting kit-8 assay for proliferation and differentiation determination.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!