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Genetic transformation in Aspergilli: tools of the trade. | LitMetric

Genetic transformation in Aspergilli: tools of the trade.

Indian J Biochem Biophys

Biotechnology Group, School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Mumbai 400 076 India.

Published: October 2004

AI Article Synopsis

  • DNA-mediated transformation enables targeted genetic modifications in organisms, particularly benefiting industries that utilize Aspergilli fungi.
  • Over the past two decades, this technique has helped address various fundamental and applied research problems in fungal biology, although there's ongoing development of new markers and strategies.
  • The review highlights the significance of these transformation systems for studying fungal gene function, with a particular focus on Aspergillus niger.

Article Abstract

DNA-mediated transformation is a powerful tool that allows the introduction of specific genetic changes in an organism. Transformation of Aspergilli, acclaimed for their wide use in the industry, has been possible for about two decades now. Several basic and applied problems related to fungal biology have been addressed using this technique. Nonetheless, new markers and strategies for transformation are still being developed for these filamentous fungi. Different methods and markers that are currently available for the transformation of Aspergilli are summarized here. The review also brings out the importance of these transformation systems in analyzing fungal gene function. Aspects of Aspergillus niger transformation are selectively emphasized.

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