AI Article Synopsis
- Tobacco viruses greatly impact the quality and yield of tobacco globally, leading to various diseases.
- A new one-step method called RT-LAMP was developed for detecting these viral diseases in tobacco, allowing for quick results in about 60 minutes.
- This RT-LAMP technique is more sensitive and specific than traditional RT-PCR, making it a reliable option for diagnosing tobacco viruses in the field.
Article Abstract
Tobacco viruses may cause a wide range of diseases that heavily reduce tobacco quality and yield worldwide. In order to detect viral diseases in tobacco fields, a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was established. Nucleotide amplification could be observed clearly after adding SYBR Green I, within 60 min under isothermal conditions, at 63-65 °C with a set of primers targeting the viral coat protein (CP) genes of tobacco viruses including cucumber mosaic virus (CMV), potato virus Y (PVY), tobacco etch virus (TEV), tobacco mosaic virus (TMV) and tobacco vein banding mosaic virus (TVBMV). This method has high specificity and sensitivity. The sensitivity of the RT-LAMP was 10 to 100 times higher than that of the conventional RT-PCR method. The RT-LAMP assay was proven reliable for virus diagnosis of tobacco samples from the field.
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| http://dx.doi.org/10.1007/s00705-012-1441-5 | DOI Listing |
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