Department of Pediatric Dentistry, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China.
Published: June 2012
AI Article Synopsis
Article Abstract
Purpose: The expression of β-catenin at the level of mRNA and protein in the apical papilla and dental pulp tissues of human permanent teeth were compared to explore the possible relationship between β-catenin and the development of pulp-dentin complex.
Methods: Forty-eight healthy permanent teeth in need of extraction for orthodontic treatment were obtained. The apical papilla and dental pulp tissues were isolated immediately when the teeth were extracted. All the samples were divided into 3 groups: group1, apical papilla of immature permanent teeth; group 2, dental pulp tissues of immature permanent teeth; group 3, dental pulp tissues of mature permanent teeth. RT-PCR and Western blot techniques were used to examine the expression of β-catenin at the level of mRNA and protein respectively. Meta Morph software was used to measure the gray value of the positively expressed bands. Two-sample t test was performed to analyze the expression intensity (gray value) of β-catenin by use of SPSS 11.0 software package.
Results: β-catenin was positively expressed at both mRNA level and protein level in all the samples investigated. The expression intensity of β-catenin in the dental pulp tissues of human immature permanent teeth was higher than that in the apical papillary tissues and that in the dental pulp tissues of human mature permanent teeth as well. The differences were statistically significant (P<0.05).
Conclusions: β-catenin was expressed in both the apical papilla and dental pulp tissues of immature and mature permanent teeth, but the expression intensity was different. It indicated that β-catenin may play an important role in the development of pulp-dentin complex and in the reparative process after the dental pulp injury.
Hereditary dentine disorders are autosomal dominant diseases that affect the development and structure of dentine, leading to various dental abnormalities and influencing the individual's oral health. It is generally classified as dentinogenesis imperfecta (DGI) and dentine dysplasia (DD). Specifically, DGI is characterized by the abnormal formation of dentine, resulting in teeth that are discolored, translucent, and prone to fracture or wear down easily.
Background: Recombinant human fibroblast growth factor-2 (rhFGF-2) has been shown to effectively promote the formation of new periodontal tissues, and its efficacy has been demonstrated in clinical settings. Moreover, the clinical and radiographic outcomes in the treatment of periodontal infrabony defects can be improved by using rhFGF-2 in combination with a bone substitute. Here, we present a case of four-wall bone defect in a tooth treated by combination regenerative therapy using rhFGF-2 and beta-tricalcium phosphate (β-TCP).
Mesenchymal stromal cells (MSCs) are a heterogeneous population of non-hematopoietic adult stem cells derived from the embryonic mesoderm. They possess self-renewal and multipotent differentiation capabilities, allowing them to give rise to mesodermal cell types, such as osteoblasts, chondroblasts, and adipocytes, as well as non-mesodermal cells, including neuron-like cells and endothelial cells. MSCs play a vital role in maintaining homeostasis across various tissues by facilitating tissue repair, immune regulation, and inflammatory response balance.
Temporomandibular joint osteoarthritis (TMJOA) is a painful inflammatory condition that limits mouth opening. Cell-derived exosomes, which have anti-inflammatory effects, are emerging as a treatment for TMJOA. Injection of dental pulp stem cells (DPSCs), which secrete exosomes, can moderate tissue damage in a rat model of TMJOA.
Dental caries is one of the most common health issues worldwide arising from the complex interactions of bacteria. In response to harmful stimuli, desirable outcome for the tooth is the formation of tertiary dentin, a protective reparative process that generates new hard tissue. This reparative dentinogenesis is associated with significant inflammation, which triggers the recruitment and differentiation of dental pulp stem cells (DPSCs).
