Unlabelled: PUPOSE: To investigate the effect of Smads signal pathway on the osteogenesis of human periodontal ligament stem cells (hPDLSCs) in simulated microgravity.
Methods: Human periodontal ligament stem cells were isolated from the ligament of surgically extracted human teeth.Through limiting dilution assay, mono-clone of the cell was obtained, hPDLSCs were isolated from MesenPRO RS medium. Rotary cell culture system (RCCS) was enrolled to set simulated microgravity (SMG). Samples were set to control group (normal gravity group, NG) and simulated microgravity group (SMG). Real-time PCR was used to detect the expression of Smads signals and the expression of markers of osteogenesis before and after SIS3. The amount of phosphated-Smad was assayed by flow cytometry. Statistical analysis of the data was done by ANOVA with SPSS 13.0 software package.
Results: Compared with that of the control group, the expression of Smads 2,3,4 was significantly higher in SMG group (P<0.05)in a time-dependent manner. Flow cytometry assay showed increased expression of p-Smads at 30-min, and peak expression was observed at 2h,reaching at 91.32%. The addition of SIS3 led to significant decrease of COL I, ALP, OCN and p-Smads (P<0.05).
Conclusion: Smads signal pathway was enrolled in the osteogenesis of hPDLSCs in simulated microgravity.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Evaluation of two low-level laser techniques as an adjunct to basic periodontal therapy: a randomized clinical trial.
Lasers Med Sci
January 2025
Federal University of Rio Grande do Norte, Natal, Brazil.
To assess and compare two techniques of low-level laser application-transgingival (TLLLT) and intrasulcular (ILLLT)-used in photobiomodulation as an adjunct to basic periodontal therapy (BPT) in patients with periodontitis. A randomized, split-mouth, double-blind clinical trial was conducted, selecting three diseased periodontal sites from different quadrants in each patient. These sites were assigned to one of three treatment groups: SRP (control), SRP + TLLLT (test 1), and SRP + ILLLT (test 2).
View Article and Find Full Text PDFAssessing periodontitis risk from specific dietary patterns: a systematic review and meta-analysis.
Clin Oral Investig
January 2025
Department of Periodontology, School of Medicine, The Second Affiliated Hospital of Zhejiang University, Hangzhou, 310009, P. R. China.
Aims: Our goal is to perform a meta-analysis to investigate the risk of periodontitis associated with specific dietary patterns.
Methods: We employed the PRISMA methodology in a meta-analysis to examine the correlation between dietary patterns and the risk of periodontitis. We systematically searched three online databases from inception to November 2024 to identify relevant studies.
Nanostructural Analysis of Age-Related Changes Affecting Human Dentin.
Calcif Tissue Int
January 2025
Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, Osaka, Japan.
Human dentin performs its function throughout life, even though it is not remodeled like bone. Therefore, dentin must have extreme durability against daily repetitive loading. Elucidating its durability requires a comprehensive understanding of its shape, structure, and anisotropy at various levels of its structure.
View Article and Find Full Text PDFBasic Science and Pathogenesis.
Alzheimers Dement
December 2024
Laboratory of Neurobiology, Department of Neurology, Poznan, Poland.
Background: Alzheimer's disease (AD) is characterized by an acquired, progressive impairment of cognitive functions. The pathogenesis of this disease remains unknown. It is explained based on the following theories: amyloid cascade, inflammation, vascular, and infection hypothesis.
View Article and Find Full Text PDFBasic Science and Pathogenesis.
Alzheimers Dement
December 2024
Glenn Biggs Institute for Alzheimer's & Neurodegenerative Diseases, The University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Background: Oral and gut microbiomes have been associated with Alzheimer's disease and related dementias (ADRD). Although the role of the gut microbiome and gut dysbiosis in ADRD has been extensively studied, research on the oral microbiome is lacking. Moreover, the synergetic contribution of oral and gut microbiomes to ADRD is unexplored.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!