Objective: The purpose of this study was to evaluate the effect of furanone as a quorum sensing inhibitor with an antibiotic agent on experimentally induced Pseudomonas aeruginosa biofilm formation in an in vitro model.
Materials And Methods: Bacterial cultures on agar plates and induction of biofilm formations were done in four different conditions; P. aeruginosa only (group 1), P. aeruginosa+ciprofloxacin (group 2), P. aeruginosa+furanone (group 3), and P. aeruginosa+furanone+ciprofloxacin (group 4). After biofilm formation on a silicone tympanostomy tube with P. aeruginosa was established, they were treated with ciprofloxacin, furanone, and ciprofloxacin with furanone respectively. All agar plates were processed for colony counts 1 day after incubation, and silicone tympanostomy tubes were observed using scanning electron microscopy 2 days after incubation. Any changes visualized on the biofilm lining the silicone tympanostomy tubes after treatment initiation were evaluated again 2 days after each treatment.
Results: There was no bacterial growth on the agar plate in group 4 compared to other groups and the formations of a biofilm on the surface of silicone tympanostomy tubes in group 4 were devoid. Little change to the original biofilm overlying the silicone tympanostomy tubes after each treatment was noted.
Conclusion: This study demonstrates that furanone may have a role as a QSI and can be helpful in preventing biofilm formation with ciprofloxacin in experimentally induced P. aeruginosa biofilms based on an in vitro model.
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