Objective: To research on the preparation of Arctigenin in vitro.
Methods: Took enzyme concentration, time course and substrate concentration as investigation factors, used Box-Behnken design-response surface methodology to optimize the enzyme hydrolysis path of Arctigenin.
Results: The best operational path for Arctigenin was as follows: the temperature was 50 degrees C, pH was 4.8, enzyme concentration was 0.44 U/mL, time course was 46.81 min, substrate concentration was 0.29 mg/mL, the conversion rate was 90.94%.
Conclusion: This research can be regarded as a referencein preparing Arctigenin in vitro.
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