Aim: The aim of this study was to evaluate the influence of different concentrations of Ethylenediaminetetraacetic acid (EDTA) solution on adhesion, that is, the bond strength of the different adhesive systems, to the pulp chamber dentin.
Materials And Methods: Recently extracted, sound, human, third molars were cut horizontally to expose the pulp horn. The roof of the pulp chamber and pulp tissue was removed. The teeth were then divided into five main groups. The teeth in each group were treated as follows: group 1, irrigated with saline; group 2, with 5% EDTA for 5 minutes; group 3, with 15% EDTA for 5 minutes; group 4, with 17% EDTA for 5 minutes and group 5, with 19% EDTA for 5 minutes. Treated specimens were dried and divided into 2 subgroups for adhesives; bonded with a total-etching adhesive (Adper Scotchbond Multi-purpose - ASB) or a one bottle of self-etch adhesive system (Clearfil S3 Bond - CS3). After the bonding procedure and composite restoration, teeth were sectioned and dentin sticks were obtained from each group for micro tensile testing (n = 10). Micro tensile testing was performed and scanning electron microscope (SEM) photographs were taken for each irrigated group.
Results: In the ASB group, saline showed statistically higher bond strength values at the different concentrations of EDTA, while the micro-tensile bond strengths of the different concentrations of EDTA were not statistically different. In the CS3 group, saline and 5% EDTA showed statistically higher bond strength values than 17% and 19% EDTA, while the micro-tensile bond strengths of 15% EDTA compared to saline and 5% EDTA and 15% EDTA compared to 17% EDTA and 19% EDTA, were not statistically different.
Conclusion: This study showed that EDTA irrigation can affect the bond strength of adhesive systems on pulp chamber lateral walls. Clinically, low EDTA concentrations can be recommended if self-etch adhesives have been selected.
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http://dx.doi.org/10.4103/0972-0707.97947 | DOI Listing |
J Phys Chem Lett
January 2025
State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
The Ni-N(His) coordination bond, formed between the nickel ion and histidine residues, is essential for recombinant protein purification, especially in Ni-NTA-based systems for selectively binding polyhistidine-tagged (Histag) proteins. While previous studies have explored its bond strength in a synthetic Ni-NTA-Histag system, the influence of the surrounding protein structure remains less understood. In this study, we used atomic force microscopy-based single-molecule force spectroscopy (AFM-SMFS) to quantify the Ni-N(His) bond strength in calprotectin, a biologically relevant protein system.
View Article and Find Full Text PDFPhys Chem Chem Phys
January 2025
Chemistry Division, Bhabha Atomic Research Centre, Mumbai, 400085, India.
The present study focuses on designing mutant peptides derived from the lanthanide binding tag (LBT) to enhance selectivity for trivalent actinide (An) ions over lanthanide (Ln) metal ions (M). The LBT is a short peptide consisting of only 17 amino acids, and is known for its high affinity towards Ln. LBT was modified by substituting hard-donor ligands like asparagine (ASN or N) and aspartic acid (ASP or D) with softer ligand cysteine (CYS or C) to create four mutant peptides: M-LBT (wild-type), M-N103C, M-D105C, and M-N103C-D105C.
View Article and Find Full Text PDFJ Chem Phys
January 2025
Department of Chemistry and Pharmaceutical Sciences, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit Amsterdam, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands, https://www.theochem.nl.
We have quantum chemically analyzed the trends in bond dissociation enthalpy (BDE) of H3C-XHn single bonds (XHn = CH3, NH2, OH, F, Cl, Br, I) along three different dissociation pathways at ZORA-BLYP-D3(BJ)/TZ2P: (i) homolytic dissociation into H3C∙ + ∙XHn, (ii) heterolytic dissociation into H3C+ + -XHn, and (iii) heterolytic dissociation into H3C- + +XHn. The associated BDEs for the three pathways differ not only quantitatively but, in some cases, also in terms of opposite trends along the C-X series. Based on activation strain analyses and quantitative molecular orbital theory, we explain how these differences are caused by the profoundly different electronic structures of, and thus bonding mechanisms between, the resulting fragments in the three different dissociation pathways.
View Article and Find Full Text PDFJ Chem Phys
January 2025
Department of Chemistry, University of Washington, Seattle, Washington 98185, USA.
We derive a new expression for the strength of a hydrogen bond (VHB) in terms of the elongation of the covalent bond of the donor fragment participating in the hydrogen bond (ΔrHB) and the intermolecular coordinates R (separation between the heavy atoms) and θ (deviation of the hydrogen bond from linearity). The expression includes components describing the covalent D-H bond of the hydrogen bond donor via a Morse potential, the Pauli repulsion, and electrostatic interactions between the constituent fragments using a linear expansion of their dipole moment and a quadratic expansion of their polarizability tensor. We fitted the parameters of the model using ab initio electronic structure results for six hydrogen bonded dimers, namely, NH3-NH3, H2O-H2O, HF-HF, H2O-NH3, HF-H2O, and HF-NH3, and validated its performance for extended parts of their potential energy surfaces, resulting in a mean absolute error ranging from 0.
View Article and Find Full Text PDFInt J Dent
January 2025
Department of Orthodontics, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
This research aimed to assess the shear bond strength (SBS) of metal brackets bonded to composite veneers using different surface preparations. One-hundred composite disks were divided into 10 different groups whereby each group combines a surface preparation (roughening or no roughening), etching agent (37% phosphoric or 9.5% hydrofluoric acid), adhesive protocol (self-etch or total-etch), and bonding agent (with or without G-Premio Bond).
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