The effects of 4-hydroxy-2-nonenal (HNE) on redox stability of Oxy- and Deoxy- wild-type (WT) and recombinant sperm whale myoglobins (P88H/Q152H, L29F, H97A, and H64F) and hemin loss from Met-myoglobin (Mb) were investigated. HNE induced greater redox instability in WT and mutant Mbs compared to controls (p < 0.05). The extent of HNE-induced OxyMb oxidation was lesser in L29F (p < 0.05) and greater in H97A and P88H/Q152H than in WT (p < 0.05). H64F DeoxyMb was more redox stable than WT DeoxyMb in the presence of HNE (p < 0.05). HNE alkylation occurred exclusively on histidine residues, and histidine 48 was alkylated in all sperm whale myoglobins. HNE alkylation accelerated the protoporphyrin moiety loss only in H97A. Met- forms of WT and L29F but not Deoxy- or Oxy- forms released hemin during storage. Primary structure strongly influenced Mb redox stability in the presence of reactive secondary lipid oxidation products.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/jf301770p | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Histochemical indications for a chemically complex signal produced by the cervical gill slit gland of the pygmy sperm whale (Kogia breviceps).
Anat Rec (Hoboken)
January 2025
Department of Biology and Marine Biology, University of North Carolina Wilmington, Wilmington, North Carolina, USA.
The pygmy sperm whale (Kogia breviceps) possesses an exocrine gland associated with its false gill slit pigmentation pattern. The cervical gill slit gland is a compound tubuloalveolar gland that produces a holocrine secretion and displays maturational changes in size and secretory histology. While the morphology of the cervical gill slit gland has been described in detail, to date, the chemical composition of its secretion remains uncharacterized.
View Article and Find Full Text PDFAn intelligent hybrid approach combining fuzzy C-means and the sperm whale algorithm for cyber attack detection in IoT networks.
Sci Rep
January 2025
Department of Information Technology Management, Faculty of Management Technology and Information System, Port Said University, Port Said, 42526, Egypt.
The Internet of Things (IoTs) has revolutionized cities, enabling them to become smarter. IoTs play an important role in monitoring the traffic cameras, roads, smart farming, connected vehicles, air quality, water level, humidity, and carbon dioxide pollution levels in city buildings. One of the major challenges of smart cities is the cyber threat to sensitive data.
View Article and Find Full Text PDFComparing neural networks against click train detectors to reveal temporal trends in passive acoustic sperm whale detections.
J Acoust Soc Am
December 2024
Sea Mammal Research Unit, School of Biology, University of St Andrews, KY16 9TH, St Andrews, United Kingdom.
Passive acoustic monitoring (PAM) is an increasingly popular tool to study vocalising species. The amount of data generated by PAM studies calls for robust automatic classifiers. Deep learning (DL) techniques have been proven effective in identifying acoustic signals in challenging datasets, but due to their black-box nature their underlying biases are hard to quantify.
View Article and Find Full Text PDFPrevious studies have described two distinct vascular systems in cetacean fins. However, these studies have been limited to Delphinoidea species, with little information on their three-dimensional structures. In this study, the anatomical analysis of the caudal and dorsal fins of a dwarf sperm whale was conducted using X-ray computed tomography and gross dissection with staining, providing the first confirmation of the two vascular systems in the fins of the family Kogiidae.
View Article and Find Full Text PDFRecords from visual surveys, strandings and eDNA sampling reveal the regular use of Reunion waters by dwarf sperm whales.
Adv Mar Biol
November 2024
BIOTOPE, Saint André, Reunion.
Article Synopsis
- The Kogia genus consists of two species: the dwarf sperm whale and the pygmy sperm whale, which are difficult to study due to their elusive behavior.
- These whales have overlapping but distinct habitat preferences, with dwarf sperm whales favoring warmer waters and pygmy sperm whales more temperate environments, and both have been noted around Reunion Island.
- Observations through various methods suggest that dwarf sperm whales are more prevalent in Reunion's waters, particularly in the northern areas, with no significant seasonal patterns identified, but their populations are vulnerable to human impacts.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!