Cell culture antigens were prepared from Vero, BHK-21 and Hep-2 cells which were propagated on slide, for detection antinuclear antibody (ANA) in sera with indirect fluorescent antibody test (IFAT). ANA were investigated in 55 sera which were positive at 1/20 titer with KB IFAT ANA test kits (Virgo), by using these cell culture antigens. 50 sera (91%) with Vero antigens, 46 sera (83%) with BHK-21 antigens and 44 sera (80%) with Hep-2 antigens were found positive at 1/20 titer. As conclusion Vero cell culture which is propagated on slide can be used as antigen for detection of ANA with IFAT.
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