We report an automated synthesis of [(18)F]-FMISO utilizing a prototype microfluidic radiochemistry module. The instrument allows for production of the tracer with 58%±2% (11 runs) decay corrected yield. Total time of production, including synthesis and purification averages 60 min. Use of the microfluidic platform results in a specific activity of 138.6 GBq/μ mol, which is higher than previously reported for conventional reactors.
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http://dx.doi.org/10.1016/j.apradiso.2012.05.022 | DOI Listing |
Biosensors (Basel)
December 2024
Department of Mechanical and Mechatronics Engineering, University of Waterloo, 200 University Ave. West, Waterloo, ON N2L 3G1, Canada.
Over the past few years, nanoplasmonic biosensors have gained widespread interest for early diagnosis of diseases thanks to their simple design, low detection limit down to the biomolecule level, high sensitivity to even small molecules, cost-effectiveness, and potential for miniaturization, to name but a few benefits. These intrinsic natures of the technology make it the perfect solution for compact and portable designs that combine sampling, analysis, and measurement into a miniaturized chip. This review summarizes applications, theoretical modeling, and research on portable nanoplasmonic biosensor designs.
View Article and Find Full Text PDFBiosensors (Basel)
November 2024
Department of Electrical and Computer Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada.
In this paper, we present a microfluidic flow cytometer for simultaneous imaging and dielectric characterization of individual biological cells within a flow. Utilizing a combination of dielectrophoresis (DEP) and high-speed imaging, this system offers a dual-modality approach to analyze both cell morphology and dielectric properties, enhancing the ability to analyze, characterize, and discriminate cells in a heterogeneous population. A high-speed camera is used to capture images of and track multiple cells in real-time as they flow through a microfluidic channel.
View Article and Find Full Text PDFBiosens Bioelectron
December 2024
Laboratory of Advanced Biotechnologies for Health Assessments (Lab-HA), Lassonde School of Engineering, York University, Toronto, ON, M3J 1P3 Canada; Department of Electrical Engineering and Computer Science (EECS), Lassonde School of Engineering, York University, Toronto, ON, M3J 1P3, Canada. Electronic address:
Rapid, point-of-care tests are critical for early diagnosis of disease and detection of biological threats. Lateral flow immunoassays (LFIAs) are well-suited for point-of-care testing due to their ease of use and straightforward readout. However, limitations in sensitivity, quantification, and integration into sample-to-result systems indicate the need for further advancements.
View Article and Find Full Text PDFBiosens Bioelectron
December 2024
Department of Biotechnology, National Formosa University, No. 64, Wunhua Rd, Huwei Township, Yunlin County, 63201, Taiwan. Electronic address:
The EZ DEVICE is an integrated fluorescence microflow cytometer designed for automated cell phenotyping and enumeration using artificial intelligence (AI). The platform consists of a laser diode, optical filter, objective lens, CMOS image sensor, and microfluidic chip, enabling automated sample pretreatment, labeling, and detection within a single compact unit. AI algorithms segment and identify objects in images captured by the CMOS sensor at 532 and 586 nm emission wavelengths.
View Article and Find Full Text PDFAdv Sci (Weinh)
December 2024
Control and Manipulation of Microscale Living Objects, Center for Translational Cancer Research (TranslaTUM), Munich Institute of Biomedical Engineering (MIBE), Department of Electrical Engineering, School of Computation, Information and Technology (CIT), Technical University of Munich, Einsteinstraße 25, 81675, Munich, Germany.
Microparticle-templated droplets or dropicles have recently gained interest in the fields of diagnostic immunoassays, single-cell analysis, and digital molecular biology. Amphiphilic particles have been shown to spontaneously capture aqueous droplets within their cavities upon mixing with an immiscible oil phase, where each particle templates a single droplet. Here, an amphiphilic microparticle with four discrete hydrophilic patches embedded at the inner corners of a square-shaped hydrophobic outer ring of the particle (4C particle) is fabricated.
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