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A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors. | LitMetric

A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors.

Biol Proced Online

System Biosciences (SBI), 265 North Whisman Road, Mountain View CA 94043, USA.

Published: August 2012

AI Article Synopsis

Article Abstract

Background: Transcription activator-like effectors (TALEs) are a class of naturally occurring transcription effectors that recognize specific DNA sequences and modulate gene expression. The modularity of TALEs DNA binding domain enables sequence-specific perturbation and offers broad applications in genetic and epigenetic studies. Although the efficient construction of TALEs has been established, robust functional tools to assess their functions remain lacking.

Results: We established a dual reporter system that was specifically designed for real-time monitoring and quantifying gene expression mediated by TALEs. We validated both sensitivity and specificity of this dual-reporter system in mammalian cells, and demonstrated that this dual reporter system is robust and potentially amenable to high throughput (HTP) applications.

Conclusion: We have designed, constructed and validated a novel dual reporter system for assessing TALE mediated gene regulations. This system offers a robust and easy-to- use tool for real-time monitoring and quantifying gene expression in mammalian cells.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509018PMC
http://dx.doi.org/10.1186/1480-9222-14-8DOI Listing

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