Unplugged is a school prevention programme widely implemtend in Europe, with some positive evaluations. This research aims to measure the impact of this program on tobacco use by means of the lifetime and last-30-day tobacco use prevalence indicators and verify the duration of the intervention's measurable effect over time. The study was designed as a randomised controlled prevention trial. The intervention is based on the Comprehensive Social Influence model and consists of 12 lessons delivered to Czech adolescents in the 2007-2008 academic year. The prevalence indicators were calculated to assess the differences between the experimental (N = 914) and control (N = 839) groups on each outcome 1, 3, 12, 15, and 24 months after the end of the intervention. Data were collected using the 2003 version of the ESPAD questionnaire. As regards the 30-day smoking prevalence indicator, the tests performed after the completion of the intervention showed statistically significant differences between both groups in favour of the experimental one. Two years after the completion of the intervention the experimental and control groups showed 30-day prevalence rates of 26.7% and 33.1%, respectively (p = .01). The progression of smoking in the 30-day prevalence among the experimental group was significantly slower than that among the control group over the period of time. The differences in the lifetime prevalence rates were not statistically significant. The implementation of Unplugged resulted in a statistically significant measurable positive effect on tobacco use in Czech adolescents.
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Heliyon
December 2024
Institute of Archaeology, Faculty of Arts, University of South Bohemia in České Budějovice, Branišovská 31a, CZ, 370 05, České Budějovice, Czech Republic.
Following European exploration of the Americas in the late 15th century, new plants rapidly spread across Europe. Simultaneously, plants from Asia and Africa arrived. Initially, they were grown in ornamental gardens but later became integral to major production centres, significantly transforming European agriculture.
View Article and Find Full Text PDFPhysiol Plant
December 2024
Department of Plant and Environmental Sciences, University of Copenhagen, Taastrup, Denmark.
The classic plant growth-promoting phytohormone cytokinin has been identified and established as a mediator of pathogen resistance in different plant species. However, the resistance effect of structurally different cytokinins appears to vary and may regulate diverse mechanisms to establish resistance. Hence, we comparatively analysed the impact of six different adenine- and phenylurea-type cytokinins on the well-established pathosystem Nicotiana tabacum-Pseudomonas syringae.
View Article and Find Full Text PDFEnviron Int
December 2024
Department of Analytical, Environmental and Forensic Sciences, School of Cancer & Pharmaceutical Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK. Electronic address:
New Phytol
February 2025
Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA.
Sci Rep
December 2024
Centre for Nanomaterials and Biotechnologies, Faculty of Science, Jan Evangelista Purkyně University in Ústí Nad Labem, Ústí Nad Labem, Czech Republic.
In recent years, there has been a growing interest in plant extracellular vesicles (pEVs) due to their immense potential for medical applications, particularly as carriers for drug delivery. To use the benefits of pEVs in the future, it is necessary to identify methods that facilitate their production in sufficient quantities while maintaining high quality. In this study, a comparative analysis of yields of tobacco pEV derived from apoplastic fluid, sterile calli, and suspension cultures, was performed to identify the most suitable plant material for vesicle isolation.
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