The aim of this study was to develop a biocompatible monolayer substrate based on fibrin and chitosan for in vitro culture of chondrocytes. Fibrin-chitosan composite substrates combined the proved cell adhesion properties of fibrin with the hydrophilicity and poor adhesion capacity of chitosan. Chitosan microspheres were produced by coacervation method, agglomerated within a fibrin network and subsequently crosslinked with genipin. The composite substrate was stable for 28 days of culture due to the high crosslinking density. Human chondrocytes cultured on the composite substrate were viable during the culture period. At the end of culture time (28 days) the composite substrate showed low cellular proliferation, 41% more collagen type II and 13% more production of sulfated glycosaminoglycans with respect to the amounts found at 14 days. The study revealed that dedifferentiated chondrocytes cultured in monolayer on the composite substrate can re-acquire characteristics of differentiated cells without using three-dimensional substrates or chondrogenic media.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jbm.a.34330 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Synthesis and characterizing of MgO, 58S bioactive glass and N carboxymethyl chitosan and coating composites of them on SS316L.
Int J Biol Macromol
December 2024
Department of Materials Science and Engineering, Faculty of Engineering & Technology, Tarbiat Modares Universirty, Tehran, Iran.
One of the most effective ways to solve the problems caused by the presence of steel implants in the body is to apply a coating to them. This study aims to develop and optimize composite coatings of magnesium oxide (MgO), 58S bioactive glass (BG), and N-carboxymethyl chitosan (N-CMC) on stainless steel (SS316L) substrates using the electrophoretic deposition (EPD) method. The synthesized materials were characterized using FTIR, XRD, and SEM to confirm their structure and morphology prior to coating.
View Article and Find Full Text PDFInterfacial functionalization and capillary force welding of enhanced silver nanowire-cellulose nanofiber composite electrodes for electroluminescent devices.
Int J Biol Macromol
December 2024
Plant Fiber Material Science Research Center, State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, PR China; Guangdong Provincial Key Laboratory of Plant Resources Biorefinery, Guangzhou 510640, PR China.
The development of flexible, intelligent, and lightweight optoelectronic devices based on flexible transparent conductive electrodes (FTCEs) utilizing silver nanowires (AgNWs) has garnered increasing attention. However, achieving low surface resistance, strong adhesion to the flexible substrate, low surface roughness, and green degradability remains a challenge. Here, a composite electrode combining natural polymer cellulose nanofibers (TCNFs) with AgNWs was prepared.
View Article and Find Full Text PDFTiCT MXene nanoribbons@MnO: A novel multifunctional probe for colorimetric and fluorescence dual-response sensing of trichlorfon.
Talanta
December 2024
Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China; College of Agricultural Equipment Engineering, Henan University of Science and Technology, Luoyang, 471003, China. Electronic address:
Manganese dioxide nanosheets (MnO NSs) have garnered significant attention in analytical sensing, while the majority of the previous reports suffer from a complex preparation process involving reducing agents, template or high-temperature. In this work, a novel MnO NSs decorated TiCT MXene nanoribbons (TiCTNR@MnO) composite was firstly assemblied via a facile one-step strategy and applied as a bi-signal generator to enable colorimetric and fluorescence (FL) dual-response sensing. During the assembly process, TiCTNR innovatively acted as both reductant and carrier to prevent the aggregation of MnO NSs.
View Article and Find Full Text PDFUltrathin ALD Coatings of Zr and V Oxides on Anodic TiO Nanotube Layers: Comparison of the Osteoblast Cell Growth.
ACS Appl Mater Interfaces
December 2024
Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00 Brno, Czech Republic.
The current study investigates and compares the biological effects of ultrathin conformal coatings of zirconium dioxide (ZrO) and vanadium pentoxide (VO) on osteoblastic MG-63 cells grown on TiO nanotube layers (TNTs). Coatings were achieved by the atomic layer deposition (ALD) technique. TNTs with average tube diameters of 15, 30, and 100 nm were fabricated on Ti substrates (via electrochemical anodization) and were used as primary substrates for the study.
View Article and Find Full Text PDFFully Flexible Humidity Sensor with Fast Response and High Responsivity Based on rGO/MoS for Human Respiration Monitoring and Nontouch Switches.
ACS Appl Mater Interfaces
December 2024
Advanced Research Institute of Multidisciplinary Sciences, Beijing Institute of Technology, Beijing 100081, China.
Humidity sensors have been widely used to monitor humidity in daily life, agriculture fields, and so on. However, conventional sensors are not suitable for wearable devices because of their large dimensions and rigid substrates. Hence, we report a fast response, highly sensitive, and fully flexible humidity sensor on a PI substrate based on the composite material of reduced graphene oxide (rGO)/MoS, with a response time of 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!