AI Article Synopsis
- D(4)-α-Cyano-4-hydroxycinnamic acid (D(4)-CHCA) is a newly synthesized matrix designed to improve matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) for analyzing small molecule drugs and biological compounds.
- Traditional MALDI-MS faced challenges due to interference from matrix ion clusters and fragment peaks that overshadowed low molecular weight signals, but D(4)-CHCA effectively shifts these interferences, revealing previously masked compounds.
- The successful application of D(4)-CHCA allowed for the direct imaging and analysis of various small molecules, including pharmaceuticals and neurotransmitters, from biological tissue samples.
Article Abstract
D(4)-α-Cyano-4-hydroxycinnamic acid (D(4)-CHCA) has been synthesized for use as a matrix for matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) and MALDI-MS imaging (MSI) of small molecule drugs and endogenous compounds. MALDI-MS analysis of small molecules has historically been hindered by interference from matrix ion clusters and fragment peaks that mask signals of low molecular weight compounds of interest. By using D(4)-CHCA, the cluster and fragment peaks of CHCA, the most common matrix for analysis of small molecules, are shifted by + 4, + 8 and + 12 Da, which expose signals across areas of the previously concealed low mass range. Here, obscured MALDI-MS signals of a synthetic small molecule pharmaceutical, a naturally occurring isoquinoline alkaloid, and endogenous compounds including the neurotransmitter acetylcholine have been unmasked and imaged directly from biological tissue sections.
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| http://dx.doi.org/10.1021/ac301498m | DOI Listing |
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