AI Article Synopsis
- The study evaluates the protective effects of natakalim on rat aortic vascular endothelial cells (RAVECs) under hypoxic conditions.
- Natakalim was administered in different dosages, with tests performed to assess cell survival, nitric oxide levels, and key gene expressions related to cell function.
- Results indicated that natakalim improved cell survival and countered the negative effects of hypoxia by reducing harmful gene expressions associated with increased cell permeability and adhesion.
Article Abstract
Objective: To investigate the protective effects of natakalim against rat aortic vascular endothelial cells (RAVECs) injuries induced by hypoxia and its mechanisms.
Methods: Selecting RAVECs as a cell model injured by hypoxia, these RAVECs were divided into 5 groups: i.e. control group, hypoxia group, natakalim low, medium and high group. The cell survival rate was determined by MTT assay, con was measured using Griess Assay, RT-PCR was used to examine t he expression of intercellular adhesion molecule-1 (ICAM-1), vascular endothelial growth factor (VEGF), endothelin-1 (ET-1) mRNA in RAVEC.
Results: Natakalim could reverse hypoxia-induced changes in endothelial cell function, including increased endothelial cell survival rate and level of NO concentration, significantly inhibited the hypoxia-induced endothelial ICAM-1, ET-1, VEGF mRNA expression levels increased.
Conclusion: Natakalim have protective effects on hypoxia-induced changes in endothelial cell function, increasing of permeation, excess expression of cell adhesion molecules.
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