Highly purified eosinophils can be isolated from peripheral blood by negative selection using an antibody-based magnetic negative selection protocol. The basic protocol describes a sequential fractionation of peripheral blood in which CD16+ granulocytes are enriched first from whole blood, followed by isolation of eosinophils. This technique is easy to use, fast, and highly reproducible. Support protocols describe a staining methods that can be used to evaluate the purity of eosinophils and differentiation from other leukocyte populations.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3594798 | PMC |
| http://dx.doi.org/10.1002/0471142735.im0731s98 | DOI Listing |
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