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Production of mixed-linkage beta-oligosaccharides from lichenan using immobilized Bacillus licheniformis UEB CF lichenase. | LitMetric
Lichenase from Bacillus licheniformis UEB CF was immobilized on Amberlite IR120 H. The immobilization yield and lichenase activity were 87 and 92.81 % of initial activity, respectively. The immobilized enzyme exhibited a shift in the optimal pH from 5.0 to 3.0, but the activity optimal temperature was not affected. The immobilized enzyme showed a residual activity of 50 % after five uses. It also exhibited high storage stability and retained 50 % of its initial activity after 120 days at 4 °C. The main hydrolysis products yielded from lichenan were trisaccharide and tetrasaccharide. The resulting mixed-linkage beta-oligosaccharides could be used as a special nutriment for lactic bacteria.
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